Evaluation of the Korean Isolate-1 Tachyzoite Antigen for Serodiagnosis of Toxoplasmosis.
- Author:
Eun Hee SHIN
1
;
Dong Hee KIM
;
Aifen LIN
;
Jo Woonyi LEE
;
Hyo Jin KIM
;
Myoung Hee AHN
;
Jong Yil CHAI
Author Information
- Publication Type:Brief Communication ; Research Support, Non-U.S. Gov't
- Keywords: Toxoplasma gondii; Toxoplasmosis; Korean isolate-1 (KI-1); RH strain; Serodiagnosis; Antigen
- MeSH: Adolescent; Adult; Animals; Antigens, Protozoan/*blood; Enzyme-Linked Immunosorbent Assay; Female; Humans; Korea; Latex Fixation Tests; Male; Mice; Middle Aged; Rabbits; Serologic Tests; Toxoplasmosis/blood/*diagnosis
- From:The Korean Journal of Parasitology 2008;46(1):45-48
- CountryRepublic of Korea
- Language:English
- Abstract: To evaluate the usefulness of the Korean Isolate-1 (KI-1) antigen for serodiagnosis of toxoplasmosis, antigen profiles of KI-1 tachyzoites were analyzed in comparison with RH tachyzoites by SDS-PAGE and immunoblotting. ELISA was performed on latex agglutination (LA)-positive and negative serum samples using KI-1 and RH antigens. Immunoblotting of the KI-1 antigen showed multiple antigen bands with molecular sizes of 22-105 kDa. Among them, 1 and 6 common bands were noted against a KI-1-infected and a RH-infected human serum, respectively, which represented differences in antigenic profiles between KI-1 and RH tachyzoites. However, all 9 LA-positive human sera were found positive by ELISA, and all 12 LA-negative sera were negative by ELISA; the correlation between the ELISA titers and LA titers was high (r = 0.749). Our results suggest that tachyzoites of KI-1 may be useful for serodiagnosis of human toxoplasmosis.
