Changes of Alpha1-Antitrypsin Levels in Allergen-induced Nasal Inflammation.
- Author:
Seung Youp SHIN
1
;
Gil Soon CHOI
;
Kun Hee LEE
;
Sung Wan KIM
;
Kyu Yeoun WON
;
Ju Hie LEE
;
Joong Saeng CHO
;
Hae Sim PARK
Author Information
1. Department of Otorhinolaryngology-Head and Neck Surgery, Kyung Hee University School of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Alpha1-antitrypsin;
Allergic rhinitis;
Eosinophil cationic protein;
IgA;
Nasal lavage fluid
- MeSH:
Antibodies;
Dermatophagoides pteronyssinus;
Eosinophil Cationic Protein;
Eosinophils;
Humans;
Immunoglobulin A;
Inflammation;
Leukocyte Elastase;
Nasal Lavage Fluid;
Nasal Mucosa;
Nasal Provocation Tests;
Pancreatic Elastase;
Rhinitis;
Rhinitis, Allergic, Perennial;
Sneezing
- From:Clinical and Experimental Otorhinolaryngology
2011;4(1):33-39
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVES: Alpha1-antitrypsin (AAT) is the main inhibitor of human neutrophil elastase, and plays a role in counteracting the tissue damage caused by elastase in local inflammatory conditions. The study evaluated the involvement of AAT in nasal allergic inflammation. METHODS: Forty subjects with mono-sensitization to Dermatophagoides pteronyssinus (Dpt) were enrolled. Twenty allergic rhinitis patients frequently complained of nasal symptoms such as rhinorrhea, stuffiness, sneezing, and showed positive responses to the nasal provocation test (NPT) with Dpt (Group I). The other 20 asymptomatic patients showed sensitization to Dpt but negative NPT (Group II). The levels of AAT, eosinophil cationic protein (ECP), and Dpt-specific IgA antibodies were measured in the nasal lavage fluids (NLFs), collected at baseline, 10 minutes, 30 minutes, 3 hours, and 6 hours after the NPT. Nasal mucosa AAT expression was evaluated with immunohistochemical staining from Group I and Group II. RESULTS: At baseline, only the Dpt-specific IgA level was significantly increased in the NLFs of Group I compared with Group II, while ECP and AAT levels were not significantly different between two groups. After Dpt provocation, AAT, ECP, and Dpt-specific IgA levels were significantly increased in the NLFs of Group I during the early and late responses. The protein expression level of AAT was mostly found in the infiltrating inflammatory cells of the nasal mucosa, which was significantly increased in Group I compared to Group II. CONCLUSION: The increment of AAT showed a close relationship with the activation of eosinophils induced by allergen-specific IgA in the NLFs of patients with allergic rhinitis after allergen stimulation. These findings implicate AAT in allergen-induced nasal inflammation.
