Megakaryocyte Colony Formation of Fetal Liver Cells.
- Author:
Byung O KWON
1
;
Hye Young JU
;
Chun Soo KIM
;
Dong Seok JEON
;
Jong In KIM
;
Heung Sik KIM
Author Information
1. Department of Pediatrics, Sungkyunkwan University, College of Medicine, Masan, Korea. chunsoo7@unitel.co.kr
- Publication Type:Original Article
- Keywords:
Hematopoietic stem cells;
Fetal liver;
CFU-Mk
- MeSH:
Bone Marrow;
Flow Cytometry;
Gestational Age;
Hematopoietic Stem Cell Transplantation;
Hematopoietic Stem Cells;
Intercellular Signaling Peptides and Proteins;
Liver*;
Megakaryocytes*;
Pancytopenia;
Stem Cells;
Thrombopoietin
- From:Journal of the Korean Pediatric Society
2002;45(2):247-255
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: This study was undertaken to obtain basic data about the megakaryocyte colony formation of fetal liver cells by using immunocytochemical staining and ex vivo culture with growth factors. METHODS: The mononuclear cells were isolated from fetal liver and bone marrow with idiopathic thrombocytopenic purpura(ITP) and pancytopenia. These mononuclear cells were cultured in MegaCultTM-C(Stem Cell Tech, Canada) media in the presence of growth factors and CFU-Megakaryocyte(CFU-Mk) colonies were counted on day 12. The expansion of CD34+ and CD41+ cell was analyzed by flow cytometry after 5 days incubation using flask culture. RESULTS: The numbers of CFU-Mk colonies of mononuclear cells obtained from fetal liver in the 11th week gestational age were more than those in the 19th week specimens; growth factors could not enhance the colony expansion in all cases. Total numbers of CFU-Mk colony of fetal liver cells were higher than bone marrow from ITP or pancytopenia groups. The numbers of pure or large CFU-Mk colonies of fetal liver cells were also higher than bone marrow specimens. The rate of CD34+ cell expression of fetal liver was increased after flask culture and the enhancement effect of epression was seen only in cases which added thrombopoietin. The rate of CD41+ cell expression of fetal liver was increased after incubation, but the enhancement effect of growth factors was unclear. CONCLUSION: This study revealed good results about the megakaryocyte colony assay of fetal liver mononuclear cells using MegaCultTM-C media. This study suggests that the fetal liver could be a good source of megakaryocytic progenitor cells for clinical application in hematopoietic stem cell transplantation.
