IL-13 R110Q, a Naturally Occurring IL-13 Polymorphism, Confers Enhanced Functional Activity in Cultured Human Bronchial Smooth Muscle Cells.
10.4168/aair.2013.5.6.377
- Author:
Ya Fang HE
1
;
Li HUA
;
Yi Xiao BAO
;
Quan Hua LIU
;
Yi CHU
;
Ding Zhu FANG
Author Information
1. Department of Pediatrics, Xinhua Hospital affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China. dr.smilebao@yahoo.com.cn
- Publication Type:Original Article
- Keywords:
IL-13;
polymorphisms;
bronchial smooth muscle cells;
functional activity
- MeSH:
Actins;
Asthma;
Calreticulin;
Enzyme-Linked Immunosorbent Assay;
Humans;
Immunoglobulin E;
Interleukin-13;
Interleukins;
Muscle, Smooth;
Myocytes, Smooth Muscle;
Myosin Heavy Chains;
Proteins
- From:Allergy, Asthma & Immunology Research
2013;5(6):377-382
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Interleukin (IL)-13, a Th2-type cytokine, plays a pivotal role in the pathogenesis of asthma through its direct effects on airway smooth muscles. A naturally occurring IL-13 polymorphism, R110Q, is strongly associated with increased total serum IgE levels and asthma. In the present study, we aimed to determine whether the IL-13 R110Q variant would display different biochemical properties or altered functions in comparison with wild-type (WT) IL-13 in cultured human bronchial smooth muscle cells (hBSMCs). METHODS: Culture supernatants and cell proteins were collected from cultured hBSMCs that were treated with 50 ng/mL IL-13 or IL-13 R110Q for 24 hours. Eotaxin released into hBSMC culture medium was determined by ELISA. The expression levels of the high-affinity IgE receptor (FcepsilonRI) alpha-chain, smooth muscle-specific actin alpha chain (alpha-SMA), smooth muscle myosin heavy chain (SmMHC), and calreticulin in the cells were measured on Western blots. RESULTS: Compared with WT IL-13, treatment with the IL-13 R110Q variant resulted in a significant increase in eotaxin release as well as significant, although modest, increases in the expression levels of alpha-SMA, SmMHC, calreticulin, and FcepsilonRI alpha-chain. CONCLUSIONS: The results of the present study suggenst that the IL-13 R110Q variant may enhance enhanced functional activities in hBSMCs.
