The Expression of Insulin-like Growth Factor (IGF), IGF- Binding Protein (IGFBP) and the Role of IGFBP-3 in the Korean Gastric Cancer Cell Lines.
- Author:
Dae Yeol LEE
1
;
Ho Keun YI
;
Doo Hyun YANG
;
Pyung Han HWANG
Author Information
1. Department of Pediatrics, Chonbuk National University Medical School, Jeonju, Korea.
- Publication Type:Original Article
- Keywords:
Insulin-like growth factor;
Insulin-like growth factor-binding protein;
Gastric cancer cell line;
Anti-proliferative agent
- MeSH:
Adenocarcinoma;
Carrier Proteins*;
Cell Line*;
Cell Survival;
Culture Media, Conditioned;
Humans;
Insulin-Like Growth Factor Binding Protein 2;
Insulin-Like Growth Factor Binding Protein 3*;
Insulin-Like Growth Factor Binding Protein 4;
Insulin-Like Growth Factor Binding Proteins;
Insulin-Like Growth Factor I;
Mitogens;
RNA, Messenger;
Stomach Neoplasms*;
Up-Regulation
- From:Journal of the Korean Cancer Association
2001;33(2):121-129
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Insulin-like growth factor (IGF)-I and II are potent mitogens, postulated to exert autocrine and paracrine effects on growth regulation in human gastric cancer. In this study, we evaluated the expression of IGF-I, -II and IGFBPs in a panel of human gastric cancer cell lines. We also evaluated whether high expression of IGFBP-3 in human gastric cancer cells may increase the sensitivity to the anti-proliferative agents. MATERIALS AND METHODS: 10 human korean gastric cancer ceIl lines and 1 Caucacian gastric adenocarcinoma cell line were used for this study. IGF and IGFBP expressions were evaluated by RT-PCR. IGFBP proteins in conditioned media were detected by Western Ligand Blot. Cell survival after treatment of anti-proliferative agents was assessed by MTT assay. RESULTS: IGF-I and II were expressed in all gastric cancer cell lines. In addition, IGF-I and II stimulated the proliferation of gastric cancer cells. The expression of IGFBP-2 was found in all gastric cancer cell lines. IGFBP-4 was expressed in the most of cell lines. IGFBP-3, -4 and -6 were expressed in about 50% of cell lines. The growth inhibition of IGFBP-3 expressing cells by anti- proliferative agents was more significant than that of IGFBP-3 nonexpressing cells. Cell growth inhibition with treatment of these agents was accompanied by increased IGFBP-3 mRNA level. CONCLUSION: These data confirm that IGF-I, -II, and certain IGFBPs were expressed in gastric cancer cells, and gastric cancer cells show the differential growth inhibition by anti-proliferative agents. The differential growth inhibitory effect of anti-proliferative agents is, at least in part, mediated through up-regulation of IGFBP-3 expression.
