Morphometric Analysis of Tibial Bone in Three Strains of Mice Using Micro-computed Tomography.
10.5625/lar.2010.26.3.315
- Author:
Byung Su AHN
1
;
Joong Sun KIM
;
Chang Geun LEE
;
Miyoung YANG
;
Changjong MOON
;
Jong Choon KIM
;
Uhee JUNG
;
Sung Ho KIM
Author Information
1. College of Veterinary Medicine, Chonnam National University, Gwangju, Korea. shokim@chonnam.ac.kr
- Publication Type:Letter
- Keywords:
Micro-computed tomography;
trabecular bone;
microarchitecture;
mouse strain
- MeSH:
Animals;
Anisotropy;
Bone Density;
Calibration;
Mice;
Mice, Inbred ICR;
Tibia
- From:Laboratory Animal Research
2010;26(3):315-318
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
This study investigated the trabecular and cortical bone microarchitecture of tibia in 14-week-old C3H/HeN, C57BL/6J and ICR mice using micro-computed tomography (micro-CT). Defined volumes of interest were scanned at a resolution of 17 micrometer (isotropic). The X-ray tube was set at photon energy of 50 kV, current of 200 microA, exposure time 1.2 sec, and a 0.5 mm-thick aluminium filter. For quantification of bone mineral density (BMD), the bone samples were scanned by micro-CT together with 2 calibration phantoms. The image slices were reconstructed using 3-dimensional CT analyzer software. C3H/HeN mice showed significantly higher levels of bone volume fraction, trabecular number and BMD, and lower levels of trabecular separation, structure model index and degree of anisotropy compared to C57BL/6J or ICR mice in trabecular bone area. So the C3H/HeN mouse appeared to be a good model animal for the study on the changes of trabecular bone with high trabecular bone mass.
