Focal Cerebral Ischemia Induces Decrease of Astrocytic Phosphoprotein PEA-15 in Brain Tissue and HT22 Cells.
10.5625/lar.2010.26.3.311
- Author:
Phil Ok KOH
1
Author Information
1. Department of Anatomy, College of Veterinary Medicine and Research Institute of Life Sciences, Gyeongsang National University, Jinju, Korea. pokoh@gnu.ac.kr
- Publication Type:Letter
- Keywords:
HT22 cells;
middle cerebral artery occlusion;
PEA-15
- MeSH:
Adult;
Animals;
Apoptosis;
Astrocytes;
Blotting, Western;
Brain;
Brain Ischemia;
Cell Death;
Cell Line;
Down-Regulation;
Glutamic Acid;
Humans;
Infarction, Middle Cerebral Artery;
Male;
Rats
- From:Laboratory Animal Research
2010;26(3):311-314
- CountryRepublic of Korea
- Language:English
-
Abstract:
PEA-15 is a small phosphoprotein (15 kDa) that is enriched in brain astrocytes. PEA-15 acts as an important modulator of cellular function including apoptosis and signal integration. This study investigated the expression of PEA-15 in focal cerebral ischemic injury. Cerebral ischemia was surgically induced in adult male rats by middle cerebral artery occlusion (MCAO), and brains were collected 24 hr after MCAO. A proteomic approach demonstrated decreases of PEA-15 protein spots in MCAO-operated animals in comparison to sham-operated animals. Western blot analysis clearly demonstrated that MCAO induces decreases in PEA-15 levels. We previously showed that glutamate toxicity induces cell death in a hippocampus-derived cell line (HT22). Glutamate exposure induces decreases of PEA-15 levels in HT22 cells. The results of this study suggest that focal cerebral ischemia induces cell death through downregulation of PEA-15 protein.
