Role of Ascorbic Acid Against the Oxidative Stress in Trabecular Meshwork Cells.
10.3341/jkos.2008.49.12.1989
- Author:
Ji Yong RYU
1
;
Jae Woo KIM
Author Information
1. Department of Ophthalmology, Catholic University of Daegu College of Medicine, Daegu, Korea. jwkim@cu.ac.kr
- Publication Type:Original Article
- Keywords:
Ascorbic acid;
Hydrogen peroxide;
Nitric oxide;
Oxidative stress;
Trabecular meshwork cells
- MeSH:
Ascorbic Acid;
Flow Cytometry;
Humans;
Hydrogen;
Hydrogen Peroxide;
Nitric Oxide;
Oxidative Stress;
Trabecular Meshwork
- From:Journal of the Korean Ophthalmological Society
2008;49(12):1989-1995
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To investigate the role of L-ascorbic acid (LAA) on the survival and its association with nitric oxide (NO) production against hydrogen peroxide-induced oxidative stress in trabecular meshwork (TM) cells. METHODS: Primarily cultured human TM cells were exposed to hydrogen peroxide; 10, 100 micrometer for 2 days, or 1 mM single exposure with or without co-exposure of LAA. Cellular survival and nitrite production were assessed with MTT and Griess assays, respectively. Flow cytometry using annexin/PI double staining was performed to evaluate apoptosis. RESULTS: Hydrogen peroxide decreased cellular survival significantly in a dose-dependent manner accompanied with decreased NO production. However cellular survival and NO production were increased significantly with co-exposure of LAA. Cellular survival and NO were highly correlated. Flow cytometric analysis revealed that LAA inhibited hydrogen peroxide-induced apoptosis. CONCLUSIONS: LAA demonstrated a cytoprotective effect against the hydrogen peroxide-induced oxidative stress accompanied with increased NO production. The cytoprotective effect of LAA may be mediated by preserving NO in TM cells.
