Age - Related Changes of Glycosaminoglycan in Senescence - Accelerated Mouse ( SAM ) Skin.
- Author:
Seung Hoon CHA
;
Seung Hoon KIM
;
Young Ho WON
;
Young Kon KIM
;
Young Pio KIM
;
Inn Ki CHUN
- Publication Type:Original Article
- Keywords:
Bullous Pemphigoid;
Epidermolysis Bullosa Acquisita;
Pattern of Fluorescence
- MeSH:
Aging*;
Alcian Blue;
Animals;
Dermis;
Electrophoresis, Polyacrylamide Gel;
Epidermolysis Bullosa Acquisita;
Humans;
Infant;
Methylene Blue;
Mice*;
Pemphigoid, Bullous;
Skin*;
Sodium
- From:Korean Journal of Dermatology
1994;32(4):631-639
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The various manifestations of intrinsic cutaneous aging may reflect the age-related changes of dermal ground aubstances, the important components of the dermis. OBJECTIVE: This study was undertaken to observe whether there is any age-relsted change, quantitatively and qualitatively, in dermal glycosaminoglycan(GAG) METHODS: The Senescence-A.ccelerated-Mice(SAM) strain "Prone" to develop senescence(P/8), aged 4 days(P-Y a group, n=5) and 4 months old (P-0 group, n=5) were used, with SAM strain Resistance to senescence (R/1), aged 4 montha old (R-0 group, n=5). The whole skin of SAM was incubated in 0.1 sodium phosphate buffer(NaPB), 2M guanidine-HC1, and 4M guanidine-HCl, sequencially, for the extractiop of GAG. The amount of GAG was measured by using alcian blue and by methylene blue metachrometic assay. Uronic acid(UA) was estimate l employing carbazole reaction. Extracted skin protein profiles were analyed by SDS-PAGE. RESULTS: 1. The total contents of GAG per wet weight of skin, as measured uairq, alcian blue, was highter in R-0 group than R-Y and P-Y group, which is statistically significant. 2. The methylene blue metaehromatic assay yielded highter absorbance values in 2M guanidine-HCl extract than NaPB ext:racts. 3. The total contents of UA decreased with aging in R strains, buttriking increase was noted in the P strain. 4. On SDS-PAGE, the protein profiles of NaPB extracts showed similarity to serum protein. 125 kDa protein band was noted only in guanidine-HCl extracts. 37.40kDa protein bands were appeared in 2M, 4M guanicline-HCl extract of R-0 group. But. there was no significant difference in both strains. CONCLUSION: These findings suggest that macromolecules, such as GAG, is one of the target molecules of the cutaneous eging process, and these change muy be related to the age-related changes of dermal water content.
