Topical Application of Eupatilin Ameliorates Atopic Dermatitis-Like Skin Lesions in NC/Nga Mice.
- Author:
Ji Hyun LEE
1
;
Ye Jin LEE
;
Jun Young LEE
;
Young Min PARK
Author Information
- Publication Type:Original Article
- Keywords: Atopic dermatitis; Dermatitis; Eupatilin; Interleukin-4; NC/Nga mice
- MeSH: Animals; Artemisia; Dermatitis; Dermatitis, Atopic; Ear; Flavonoids; Inflammation; Interleukin-4; Mast Cells; Mice*; Necrosis; Pruritus; Skin*; Therapeutic Uses
- From:Annals of Dermatology 2017;29(1):61-68
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: Atopic dermatitis (AD) is an inflammatory skin disorder with severe pruritus. Despite advancements in medicine, therapeutic treatments for AD are still limited. Eupatilin (5,7-dihydroxy-30,40,6-trimethoxyflavone) is one of the lipophilic flavonoids from Artemisia umbelliformis Lam. and Artemisia genipi Weber. OBJECTIVE: Although it has been reported to act a role in improving inflammation, its action on AD is uncertain. In this study, we examined the role of eupatilin on AD-like skin lesions in NC/Nga mice. METHODS: 2,4-dinitrochlorobenzene was repeatedly applied to the ear of NC/Nga mice to produce AD-like skin lesions. Eupatilin (1%, once a day for 5 consecutive days/week) was applied topically for four weeks for the evaluation of its therapeutic effects. RESULTS: 1% eupatilin cream significantly reduced the clinical severity score of AD-like lesions, compared to the vehicle (p<0.005). A histopathological analysis revealed that 1% eupatilin cream significantly decreased the mast cell infiltration as well as inflammatory cell infiltration, compared to the vehicle (p<0.005). We showed that 1% eupatilin cream significantly reduced the expression of thymic stromal lymphopoietin, tumor necrosis factor-α, interleukin-4, and interleukin-19, but not interferon-γ, compared to the vehicle (p<0.005). CONCLUSION: Considering the therapeutic reaction of eupatilin on AD-like lesions as in this study, the substance has a promising to be an adjuvant topical agent for the control of AD.
