Activated platelets induce secretion of interleukin-1beta, monocyte chemotactic protein-1, and macrophage inflammatory protein-1alpha and surface expression of intercellular adhesion molecule-1 on cultured endothelial cells.
10.3346/jkms.2000.15.3.273
- Author:
Jae Kwab CHA
1
;
Min Ho JEONG
;
Hae Rahn BAE
;
Jin Young HAN
;
Soo Jin JEONG
;
Hyun Ju JIN
;
Young Jin LIM
;
Sang Ho KIM
;
Jae Woo KIM
Author Information
1. Department of Neurology, School of Medicine, Dong-A University, Pusan, Korea. nrcjk@unitel.co.kr
- Publication Type:Original Article
- Keywords:
Blood Platelets;
Endothelium;
Cell Adhesion Molecules;
Atherosclerosis
- MeSH:
Blood Platelets/metabolism*;
Cells, Cultured;
Cells, Cultured;
Endothelium, Vascular/secretion*;
Endothelium, Vascular/cytology;
Human;
Intercellular Adhesion Molecule-1/biosynthesis;
Interleukin-1/secretion*;
Macrophage Inflammatory Protein-1/secretion*;
Monocyte Chemoattractant Protein-1/secretion*;
Platelet Activation/physiology*
- From:Journal of Korean Medical Science
2000;15(3):273-278
- CountryRepublic of Korea
- Language:English
-
Abstract:
Atherosclerosis is an inflammatory disease. Platelet-endothelium interaction plays an important role in the pathophysiology of atherogenesis. We investigated the role of activated platelets for secretion of interleukin (IL)-1beta, monocyte chemotactic protein (MCP)-1 and macrophage inflammatory protein (MIP)-1alpha and expression of intercellular adhesion molecule (ICAM)-1 on endothelial cells. Human umbilical vein endothelial cells (HUVEC) were incubated with non-stimulated or ADP-activated platelets for 6 hr. Secretion of interleukin (IL)-1beta, MCP-1 and MIP-1alpha and surface expression of ICAM-1 were measured by ELISA and flow cytometry. In the presence of activated platelets, the secretion of IL-1beta, MCP-1, and MIP-1alpha and surface expression of ICAM-1 were significantly increased compared with non-activated platelets. The present study shows that activated platelets may contribute to expression of various inflammatory mediators on endothelial cells.
