Matrix metalloproteinases in human gliomas: activation of matrix metalloproteinase-2 (MMP-2) may be correlated with membrane-type-1 matrix metalloproteinase (MT1-MMP) expression.
10.3346/jkms.2000.15.3.309
- Author:
Jin Heang HUR
1
;
Myung Jin PARK
;
In Chul PARK
;
Dong Hee YI
;
Chang Hun RHEE
;
Seok Il HONG
;
Seung Hoon LEE
Author Information
1. Laboratory of Cell Biology, Korea Cancer Center Hospital, Seoul.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Glioma;
Matrix Metalloproteinase (MMPs);
Tissue Inhibitor of Metalloproteinase (TIMPs);
Membrane-Type-1 Metalloproteinase (MT1-MMP);
Neoplasms Invasiveness
- MeSH:
Animal;
Blotting, Northern/methods;
Brain/pathology;
Brain Neoplasms/pathology;
Brain Neoplasms/enzymology*;
Enzyme Activation;
Gelatinase A/metabolism;
Gelatinase A/genetics*;
Gelatinase B/metabolism;
Gene Expression Regulation, Enzymologic*;
Glioma/pathology;
Glioma/enzymology*;
Human;
Metalloendopeptidases/genetics*;
Papio;
Tissue Inhibitor-of Metalloproteinase-2/genetics;
Tissue-Inhibitor of Metalloproteinase-1/genetics;
Tumor Cells, Cultured
- From:Journal of Korean Medical Science
2000;15(3):309-314
- CountryRepublic of Korea
- Language:English
-
Abstract:
To evaluate possible roles of matrix metalloproteinase (MMP)-1, -2, tissue inhibitor of metalloproteinase (TIMP)-1, -2 and membrane-type-1 matrix metalloproteinase (MT1-MMP) in invasion of human gliomas, expressions of these proteins were investigated in ten cases of human glioma and two meningioma tissues and eight human glioma cell lines. In gelatin zymography, MMP-2 activities of glioblastomas were higher than astrocytomas. The activated form of MMP-2 was seen in five of six cases of glioblastomas, but not in astrocytomas. MMP-9 activity was detected in all cases of malignant astrocytomas but the reactivity of MMP-9 was weaker than that of MMP-2. MT1-MMP mRNA expression in glioblastomas was higher than that in astrocytomas. Five cases of glioblastomas with activated form of MMP-2 had MT1-MMP expressions. In vitro, human glioma cell lines with high expression of MT1-MMP also showed high MMP-2 activity. TIMP-1 transcripts were constitutively present in almost all glioma tissues and cell lines, whereas TIMP-2 mRNA were weak especially in malignant gliomas. Imbalance of TIMP-2/MMP-2 was observed using immunoprecipitation analysis in a glioma cell line. High expression of MMP-2 and MT1-MMP is possibly involved in invasiveness of malignant glioma.
