Easy Application of Digoxigenin-11-dUTP Labelled Probe in Detection of Human Papillomavirus DNA.
10.5021/ad.1991.3.2.91
- Author:
Kyang Joon PARK
;
Ho Suk SUNG
- Publication Type:Original Article
- Keywords:
In situ hybridization;
Condyloma acuminatum;
Digoxigenin-11-dUTP;
Radioactive isotope
- MeSH:
DNA*;
Humans*;
In Situ Hybridization
- From:Annals of Dermatology
1991;3(2):91-95
- CountryRepublic of Korea
- Language:English
-
Abstract:
In situ hybridization was performed in ten cases of condyloma acuminata in order to study the applicability of digoxigenin-11 dUTP(Dig-dUTP) labelled probe compared with radioactive isotope labelled probes. Although signal intensity was denser in radiolabelled probes, high positive rates were obtained with Dig-dUTP labelled probes. From these results, Dig-dUTP labelling is found to be more efficient in typing of human papillomavirus DNA than radiolabelling.
