Specific B-cell Epitope of Per a 1: A Major Allergen of American Cockroach (Periplaneta americana) and Anatomical Localization.
10.4168/aair.2014.6.4.325
- Author:
Nitat SOOKRUNG
1
;
Thanyathon KHETSUPHAN
;
Urai CHAISRI
;
Nitaya INDRAWATTANA
;
Onrapak REAMTONG
;
Wanpen CHAICUMPA
;
Anchalee TUNGTRONGCHITR
Author Information
1. Office for Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
- Publication Type:Original Article
- Keywords:
American cockroach;
B-cell epitope;
hybridoma;
Per a 1;
Periplaneta americana, mimotope
- MeSH:
Allergens;
Amino Acid Sequence;
Animals;
Antibodies, Monoclonal;
Bacteriophages;
Cockroaches;
Epitopes, B-Lymphocyte*;
Feces;
Gastrointestinal Contents;
Hybridomas;
Immunoglobulin E;
Mice;
Peptides;
Periplaneta*;
Protein Isoforms
- From:Allergy, Asthma & Immunology Research
2014;6(4):325-332
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Cockroach (CR) is a common source of indoor allergens, and Per a 1 is a major American CR (Periplaneta americana) allergen; however, several attributes of this protein remain unknown. This study identifies a novel specific B cell epitope and anatomical locations of Per a 1.0105. METHODS: Recombinant Per a 1.0105 (rPer a 1.0105) was used as BALB/c mouse immunogen for the production of monoclonal antibodies (MAb). The MAb specific B cell epitope was identified by determining phage mimotopic peptides and pair-wise alignment of the peptides with the rPer a 1.0105 amino acid sequence. Locations of the Per a 1.0105 in P. americana were investigated by immunohistochemical staining. RESULTS: The rPer a 1.0105 (~13 kDa) had 100%, 98% and > or =90% identity to Per a 1.0105, Per a 1.0101, and Cr-PII, respectively. The B-cell epitope of the Per a 1.0105 specific-MAb was located at residues99 QDLLLQLRDKGV110 contained in all 5 Per a 1.01 isoforms and Per a 1.02. The epitope was analogous to the Bla g 1.02 epitope; however, this B-cell epitope was not an IgE inducer. Per a 1.0105 was found in the midgut and intestinal content of American CR but not in the other organs. The amount of the Per a 1 was ~544 degrees Cg per gram of feces. CONCLUSIONS: The novel Per a 1 B-cell epitope described in this study is a useful target for allergen quantification in samples; however, the specific MAb can be used as an allergen detection reagent. The MAb based-affinity resin can be made for allergen purification, and the so-purified protein can serve as a standard and diagnostic allergen as well as a therapeutic vaccine component. The finding that the Per a 1 is contained in the midgut and feces is useful to increase yield and purity when preparing this allergen.
