Use of RT-PCR for CK20 and CEA mRNA to Detect of Micrometastasis in the Draining Venous Blood and the Peritoneum in Gastric Cancer Patients.
10.5230/jkgca.2003.3.3.128
- Author:
Jai Kyun JOO
1
;
Ji Hee LEE
;
Yang Seok KOH
;
Jung Chul KIM
;
Seong Yeob RYU
;
Dong Yi KIM
;
Young Jin KIM
Author Information
1. Divisin of Gastroenterology, Departments of Surgery, College of Medicine Chonnam National University Hospital, Gwangju, Korea. kimyjin@jnu.ac.kr
- Publication Type:Original Article
- Keywords:
Gastric cancer;
Micrometastasis;
Reverse transcriptase polymerase chain reaction (RT-PCR);
Carcinoembryonic antigen (CEA);
Cytokeratin 20 (CK-20)
- MeSH:
Carcinoembryonic Antigen;
Douglas' Pouch;
Gastrointestinal Neoplasms;
Humans;
Keratin-20;
Neoplasm Micrometastasis*;
Peritoneum*;
Reverse Transcriptase Polymerase Chain Reaction;
RNA, Messenger*;
Stomach Neoplasms*
- From:Journal of the Korean Gastric Cancer Association
2003;3(3):128-133
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The benefits of the "no-touch" isolation technique that is usually performed to prevent the circulation of tumor cells are not evident. The aim of this study was to determine whether the no-touch isolation technique for treating gastrointestinal cancers could prevent the circulation of tumor cells detected by reverse transcriptase polymerase chain reaction (RT-PCR). Matrials and Methods: By using RT-PCR to amplify mRNAs for two specific epithelial markers, carcinoembryonic antigen (CEA) and cytokeratin 20 (CK-20), we examined 34 gastric cancer patients who had been histologically diagnosed and 22 patients had undergone serosal and peritoneal brushing. RESULTS: In 10 (29.4%) of the 34 gastric cancer patients, we detected CK20 mRNA before manipulation, and in 17 (51.5%) of those patients, after we detected it. The density of the CK20 mRNA band was increased in 11 cases (33.3%) and the density was decreased in 2 cases (6.1%). In 16 (48.5%) of the 34 gastric cancer patients, we detected CEA mRNA before manipulation, and in 17 (51.5%) patients after we detected it. The density of the CEA mRNA band was increased in 8 cases (24.2%) and decreased in 3 cases (9.1%). CONCLUSION: These result suggest that the "no-touch isolation technique" might be useful when operating on advanced gastric cancer patients and that serosal or Douglas pouch brushing can be used to determine the status of micrometastasis.
