Relationship between Microdeletions on the Y Chromosome and Defect of Spermatogenesis.
- Author:
Hyoung Song LEE
1
;
Hye Won CHOI
;
Yong Seog PARK
;
Mi Kyoung KOONG
;
Inn Soo KANG
;
Jong Min YUN
;
You Sik LEE
;
Ju Tae SEO
;
Jin Hyun JUN
Author Information
1. Laboratory of Reproductive Biology and Infertility, Samsung Cheil Hospital and Women's Healthcare Center, Sungkyunkwan University School of Medicine, Seoul, Korea. junjh55@hanmail.net
- Publication Type:Original Article
- Keywords:
Y chromosome;
Microdeletion;
Azoospermia factor (AZF);
Genetic counselling
- MeSH:
Azoospermia;
DNA;
Germ Cells;
Humans;
Hyperplasia;
Incidence;
Infertility;
Korea;
Male;
Oligospermia;
Phenotype;
Polymerase Chain Reaction;
Sequence Tagged Sites;
Sertoli Cell-Only Syndrome;
Spermatogenesis*;
Y Chromosome*
- From:Korean Journal of Fertility and Sterility
2002;29(4):303-310
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVES: To estimate the frequency of Y chromosome microdeletions in the Korean population of infertile men and to evaluate the relationship between microdeletion on the Y chromosome and clinical phenotypes of infertile men with idiopathic azoospermia and oligozoospermia. MATERIALS AND METHODS: Genomic DNA was extracted from blood samples collected from 330 infertile men attending the Infertility Clinic at Samsung Cheil Hospital, Korea. Six sequence tagged sites (STSs) spanning the azoospermia factor (AZF) regions of the Y chromosome were amplified by polymerase chain reactions (PCRs). RESULTS: Microdeletions on Y chromosome were detected in 35 (10.6%) of the 330 infertile men. Most of the microdeletions (91.4%) involved AZFb or AZFc. The high incidence of microdeletions were found in AZFc region (57.1%), but the low in AZFa (8.6%) and AZFb (5.7%). Larger microdeletions involving two or three AZF regions were detected in 28.6% of cases. All patients (6 patients) with deletion of AZFa region showed no germ cell phenotypes, Sertoli cell only syndrome or Leydig cell hyperplasia in histopathologic examinations. CONCLUSION: Microdeletions on the Y chromosome, especially, at AZFc/DAZ regions may be the major cause of azoospermia and severe oligozoospermia. We suggest that idiopathic infertile men have genetic counselling and microdeletion analysis on the Y chromosome before IVF-ET and ART program.
