Differential diagnosis of Trichostrongylus and hookworm eggs via PCR using ITS-1 sequence.
- Author:
Tai Soon YONG
1
;
Jong Ho LEE
;
Seobo SIM
;
Jongweon LEE
;
Duk Young MIN
;
Jong Yil CHAI
;
Keeseon S EOM
;
Woon Mok SOHN
;
Soon Hyung LEE
;
Han Jong RIM
Author Information
- Publication Type:Brief Communication ; Research Support, Non-U.S. Gov't
- Keywords: Trichostrongylus colubriformis; Ancylostoma duodenale; Necator americanus; PCR diagnosis; ITS-1
- MeSH: Ancylostoma/*genetics/isolation & purification; Ancylostomiasis/*diagnosis/parasitology; Animals; Base Sequence; DNA, Intergenic/genetics; DNA, Protozoan/genetics; DNA, Ribosomal Spacer/genetics; Diagnosis, Differential; Humans; Molecular Sequence Data; Necator americanus/*genetics/isolation & purification; Phylogeny; Polymerase Chain Reaction/*methods; Sequence Alignment; Trichostrongylosis/*diagnosis/parasitology; Trichostrongylus/*genetics/isolation & purification
- From:The Korean Journal of Parasitology 2007;45(1):69-74
- CountryRepublic of Korea
- Language:English
- Abstract: Trichostrongylus eggs observed in cellophane-thick smears are difficult, in practice, to distinguish from hookworm eggs. In order to overcome these limitations, a molecular approach was conducted. A Trichostrongylus colubriformis adult worm was obtained from a human in Laos, which was identified morphologically. ITS-1 sequence of this worm was determined, and found to be most similar with that of T. colubriformis among the Trichostrongylus spp. reported so far. Then, this sequence was compared with those of human hookworm species, Ancylostoma duodenale and Necator americanus, and species-specific oligonucleotide primers were designed. Polymerase chain reaction (PCR) using these primers evidenced specifically amplified PCR products of Trichostrongylus sp., A. duodenale and N. americanus from the eggs of each (520 bp, 690 bp, and 870 bp, respectively). A species-specific PCR technique can be developed in order to study the epidemiology of Trichostrongylus spp. and hookworms in endemic areas.
