A simple rat model of in situ reversible obstructive jaundice in situ reversible obstructive jaundice model.
10.4174/astr.2017.92.6.389
- Author:
Xin HUANG
1
;
Chong Hui LI
;
Ai Qun ZHANG
;
Zhe KONG
;
Wan Qing GU
;
Jia Hong DONG
Author Information
1. Center for Hepatopancreatobiliary Diseases, Beijing Tsinghua Changgung Hospital, Tsinghua University Medical Center, Beijing, China. dongjiahong@mail.tsinghua.edu.cn
- Publication Type:Original Article
- Keywords:
Animal model;
Drainage;
Obstructive jaundice;
Rats
- MeSH:
Animals;
Bile Ducts;
Body Weight;
Choledochostomy;
Common Bile Duct;
Drainage;
Inflammation;
Jaundice, Obstructive*;
Laparotomy;
Ligation;
Liver;
Models, Animal*;
Mortality;
Rats*
- From:Annals of Surgical Treatment and Research
2017;92(6):389-395
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: To develop a simple and reliable rat model of in situ reversible obstructive jaundice with low morbidity and mortality rates. METHODS: Rats were divided into 4 groups with 8 rats each: the sham-operated (SH) group only underwent laparotomy, the control internal drainage (ID-C) group underwent choledochoduodenostomy, the new internal drainage (ID-N) group and the long-term internal drainage (ID-L) group underwent choledochocholedochostomy. Common bile duct ligation was performed in all the drainage groups 7 days before reversal procedures. All rats were sacrificed for samples 7 days after the last operation except rats of the ID-L group that survived 28 days before sacrifice. Body weight, liver function, histopathological changes, morbidity and mortality were assessed. RESULTS: One rat died and 2 rats had complications with tube blockage in the ID-C group. No death or complications occurred in the ID-N and ID-L groups. The drainage tube remained patent in the long-term observation ID-L group. Body weight showed no significant difference between the ID-C and ID-N groups after 7 days drainage. Liver function was not fully recovered in the ID-C and ID-N groups after 7 days drainage, but statistical differences were only observed in the ID-C group compared with the SH and ID-L groups. Periportal inflammation and bile duct proliferation showed severer in the ID-C group than in the ID-N group. CONCLUSION: The present study provided an efficient, simple, and reliable rat model that is especially suitable for long-term or consecutive studies of reversible obstructive jaundice.
