Effect of Mercury Chloride on Peritoneal Macrophage or EMT-6 cell from Balb/c mice.
- Author:
Dai Ha KOH
1
;
Jung Ho YOUM
;
No Suk KI
;
Gyung Jae OH
;
Kuen Sang KWON
;
Sung Yeup KIM
;
Nam Song KIM
Author Information
1. Department of Preventive Medicine and Public Health Choabuk National University Medical School, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
Mercury chloride;
Peritoneal macrophage;
EMT-6 cell;
Nitrite;
Nitrate
- MeSH:
Animals;
Culture Media;
Cytokines;
Immunity, Cellular;
Macrophages, Peritoneal*;
Metabolism;
Mice*;
Nitric Oxide;
Survival Rate
- From:Korean Journal of Occupational and Environmental Medicine
1996;8(2):201-209
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The effect of treatment of mercury chloride on the nitrite and nitrate synthesis was observed in peritoneal macrophages from Balb/c mice and EMT-6 cells in vitro. The cells were cultured in Dulbecco's modified Eagle's medium (DMEM) with cytokines. Amounts of nitrite and nitrate in the culture media after 24 and 36 hours of culture were about 2-fold, and 3-fold of those measured after 12 hours respectively. There were very close associations between the amounts of nitrite and nitrate measured in the culture media, according to culture time. The survival rate of peritoneal macrophages was significantly decreased by mercury chloride added into the media in dose-dependent manner, however the survivals of EMT-6 cells were not influenced by mercury chloride concentration in media. Nitrite and nitrate syntheses were dose-dependently decreased by mercury chloride added in culture media. These results reported here suggest that the disorder of cell mediated immunity by mercurials could be related to the inhibition of nitric oxide synthesis which seems to be caused by the inhibition of metabolism of cells.
