Mixed Mononuclear Cell Culture of Cord Blood and Adult Peripheral Blood.
- Author:
Eun Jin CHOI
1
;
Kun Soo LEE
Author Information
1. Department of Pediatrics, Catholic University of Daegu School of Medicine, Daegu, Korea. ejchoi2@cataegu.ac.kr
- Publication Type:Original Article
- Keywords:
Mixed mononuclear cell culture;
Cord blood mononuclear cells;
Adult peripheral blood mononuclear cells;
Cord blood transplantation
- MeSH:
Adult*;
Bone Marrow Cells;
Cell Count;
Cell Culture Techniques*;
Fetal Blood*;
Hematopoietic Stem Cells;
Humans;
Tissue Donors;
Unrelated Donors
- From:Korean Journal of Pediatric Hematology-Oncology
2002;9(2):211-219
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Umbilical cord blood as the source of hematopoietic stem cells has several advantages over bone marrow cells for transplant purpose, but the limitation inherent in the cord blood is its limited number of hematopoietic stem cells. The responses of cord blood mononuclear cells (MNC) which were stimulated by unrelated cord blood MNC or adult peripheral blood MNC to investigate the possibility of unrelated, HLA-mismatched multiple cord blood transplantation were evaluated. METHODS: Nineteen cord blood and 7 adult peripheral blood samples were used. Each of the MNC from cord blood and adult peripheral blood was cultured before and after 2,000 cGy irradiation. Mixed MNC cultures with cord blood and irradiated different cord blood or irradiated adult peripheral blood were performed. RESULTS: The mean percentages of MNC counts compared to day 0 of day 2 and day 7 cultures of mixed MNC cultures with cord blood and irradiated different cord blood were 92.5 55.8%, 57.4 51.0% and those of mixed MNC cultures with cord blood and irradiated adult peripheral blood were 143.4 132.7%, 113.4 95.1%, respectively. The difference of the cell count changes in 2 types of MNC cultures was statistically significant (P<0.05). CONCLUSION: The proliferative responses of cord blood MNC to different cord blood MNC were significantly lower than those to adult peripheral blood MNC. The study for the investigation of clinical applicability of cord blood transplantation from multiple unrelated donors to overcome the limitation of the cell dose of cord blood transplantation and the shortage of related or unrelated HLA-matched donors is needed.
