Nanoliposomes of L-lysine-conjugated poly(aspartic acid) Increase the Generation and Function of Bone Marrow-derived Dendritic Cells.
- Author:
Sun A IM
1
;
Ki Hyang KIM
;
Hong Geun JI
;
Hyoung Gyoung YU
;
Sun Ki PARK
;
Chong Kil LEE
Author Information
- Publication Type:Original Article
- Keywords: Nanoliposome; Poly(aspartic acid); L-Lysine; Dendritic cell; Immunomodulation
- MeSH: Animals; Aspartic Acid; Cytokines; Dendritic Cells; Immunomodulation; Interleukin-12; Interleukin-6; Lymphocyte Culture Test, Mixed; Lysine; Mice; Polymers; T-Lymphocytes; Tumor Necrosis Factor-alpha
- From:Immune Network 2011;11(5):281-287
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: Biodegradable polymers have increasingly been recognized for various biological applications in recent years. Here we examined the immunostimulatory activities of the novel poly(aspartic acid) conjugated with L-lysine (PLA). METHODS: PLA was synthesized by conjugating L-lysine to aspartic acid polymer. PLA-nanoliposomes (PLA-NLs) were prepared from PLA using a microfluidizer. The immunostimulatory activities of PLA-NLs were examined in mouse bone marrow-derived dendritic cells (BM-DCs). RESULTS: PLA-NLs increased the number of BM-DCs when added to cultures of GM-CSF-induced DC generation on day 4 after the initiation of cultures. Examination of the phenotypic properties showed that BM-DCs generated in the presence of PLA-NLs are more mature in terms of the expression of MHC class II molecules and major co-stimulatory molecules than BM-DCs generated in the absence of PLA-NLs. In addition, the BM-DCs exhibited enhanced capability to produce cytokines, such as IL-6, IL-12, TNF-alpha and IL-1beta. Allogeneic mixed lymphocyte reactions also confirmed that the BMDCs were more stimulatory on allogeneic T cells. PLA- NL also induced further growth of immature BM-DCs that were harvested on day 8. CONCLUSION: These results show that PLA-NLs induce the generation and functional activities of BM-DCs, and suggest that PLA-NLs could be immunostimulating agents that target DCs.
