In vitro migration capacity of human adipose tissue-derived mesenchymal stem cells reflects their expression of receptors for chemokines and growth factors.
10.3858/emm.2011.43.10.069
- Author:
Sun Jin BAEK
1
;
Sung Keun KANG
;
Jeong Chan RA
Author Information
1. Stem Cell Research Center, RNL BIO Co., Ltd., Seoul 153-768, Korea. jcra@rnl.co.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
adipose tissue;
cell migration assays;
cell movement;
chemokines;
cytokines;
mesenchymal stem cells;
receptors, chemokine;
receptors, cytokine
- MeSH:
Adipose Tissue/*cytology;
Cell Movement/drug effects;
Cell Separation;
Cells, Cultured;
Flow Cytometry;
Gene Expression Regulation/drug effects;
Humans;
*Mesenchymal Stem Cell Transplantation;
Mesenchymal Stem Cells/cytology/drug effects/*metabolism;
Receptors, Chemokine/genetics/*metabolism;
Receptors, Growth Factor/genetics/*metabolism;
Tumor Necrosis Factor-alpha/pharmacology
- From:Experimental & Molecular Medicine
2011;43(10):596-603
- CountryRepublic of Korea
- Language:English
-
Abstract:
The homing properties of adipose tissue-derived mesenchymal stem cells (AdMSCs) have stimulated intravenous applications for their use in stem cell therapy. However, the soluble factors and corresponding cellular receptors responsible for inducing chemotaxis of AdMSCs have not yet been reported. In the present study, the migration capacity of human AdMSCs (hAdMSCs) toward various cytokines or growth factors (GFs) and the expression of their receptors were determined. In a conventional migration assay, PDGF-AB, TGF-beta1, and TNF-alpha showed the most effective chemoattractant activity. When AdMSCs were preincubated with various chemokines or GF, and then allowed to migrate toward medium containing 10% FBS, those preincubated with TNF-alpha showed the highest migratory activity. Next, hAdMSCs were either preincubated or not with TNF-alpha, and allowed to migrate in response to various GFs or chemokines. Prestimulation with TNF-alpha increased the migration activity of hAdMSCs compared to unstimulated hAdMSCs. When analyzed by FACS and RT-PCR methods, hAdMSCs were found to express C-C chemokine receptor type 1 (CCR1), CCR7, C-X-C chemokine receptor type 4 (CXCR4), CXCR5, CXCR6, EGF receptor, fibroblast growth factor receptor 1, TGF-beta receptor 2, TNF receptor superfamily member 1A, PDGF receptor A and PDGF receptor B at both the protein and the mRNA levels. These results indicate that the migration capacity of hAdMSCs is controlled by various GFs and chemokines. Prior in vitro modulation of the homing capacity of hAdMSCs could stimulate their movement into injured sites in vivo when administered intravenously, thereby improving their therapeutic potential.
