Prevalence and molecular characterization of Cryptosporidium spp. in dairy cattle from farms in China.
- Author:
Fu CHEN
1
;
Kehe HUANG
Author Information
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords: Cryptosporidium; dairy cattle; modified acid-fast staining; phylogenetic analysis; polymerase chain reaction
- MeSH: Animals; Base Sequence; Cattle; Cattle Diseases/epidemiology/*parasitology; Chi-Square Distribution; China/epidemiology; Cryptosporidiosis/epidemiology/parasitology/*veterinary; Cryptosporidium/genetics/*isolation & purification; DNA, Protozoan/chemistry/genetics; Feces/parasitology; Female; Molecular Sequence Data; Phylogeny; Polymerase Chain Reaction/veterinary; Prevalence; RNA, Ribosomal, 18S/chemistry/genetics; Sequence Alignment; Sequence Analysis, DNA
- From:Journal of Veterinary Science 2012;13(1):15-22
- CountryRepublic of Korea
- Language:English
- Abstract: Fecal samples of 2,056 dairy cattle from 14 farms were collected in three geographical regions of China and stained using a modified acid-fast staining technique to identify Cryptosporidium oocysts. A total of 387 (18.82%) positive samples were identified and further analyzed by polymerase chain reaction (PCR) using primers designed to amplify DNA fragments from the small subunit ribosomal RNA. The PCR products were sequenced and the sequences were deposited in the GenBank database under accession numbers EU369377-84 and GU070730-33. Phylogenetic analysis was performed and a distances matrix generated from these sequences confirmed the existence of Cryptosporidium (C.) parvum 'mouse' genotype, C. bovis, C. andersoni, C. hominis, and C. serpentis in cattle. These results represent the first report on the prevalence and genetic identification of Cryptosporidium species, and may contribute to a better understanding of the epidemiology of Cryptosporidium in cattle in China.
