Immunomodulatory effects of human amniotic membrane-derived mesenchymal stem cells.
- Author:
Jung Won KANG
1
;
Hye Cheong KOO
;
Sun Young HWANG
;
Sung Keun KANG
;
Jeong Chan RA
;
Moon Han LEE
;
Yong Ho PARK
Author Information
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords: amniotic membrane; IL-10; immune modulation; mesenchymal stem cells; TGF-beta
- MeSH: Amnion/cytology/*immunology; Cell Differentiation/immunology; Coculture Techniques; Dinoprostone/genetics/immunology; Female; Hepatocyte Growth Factor/genetics/immunology; Humans; Immunologic Factors/*immunology; Immunophenotyping; Indoleamine-Pyrrole 2,3,-Dioxygenase/genetics/immunology; Interferon-gamma/immunology; Interleukin-10/analysis/immunology; Interleukin-17/analysis/immunology; Leukocytes, Mononuclear/cytology/immunology; Mesenchymal Stem Cells/cytology/*immunology; Pregnancy; RNA, Messenger/chemistry/genetics; Regenerative Medicine/methods; Reverse Transcriptase Polymerase Chain Reaction; Transforming Growth Factor beta/genetics/immunology
- From:Journal of Veterinary Science 2012;13(1):23-31
- CountryRepublic of Korea
- Language:English
- Abstract: Human amniotic membrane-derived mesenchymal stem cells (hAM-MSCs) are capable of differentiating into several lineages and possess immunomodulatory properties. In this study, we investigated the soluble factor-mediated immunomodulatory effects of hAM-MSCs. Mitogen-induced peripheral blood mononuclear cell (PBMC) proliferation was suppressed by hAM-MSCs in a dose-dependent manner as well as hAM-MSC culture supernatant. Moreover, interferon-gamma and interleukin (IL)-17 production significantly decreased from PBMC, whereas IL-10 from PBMCs and transforming growth factor beta (TGF-beta) production from hAM-MSCs significantly increased in co-cultures of hAM-MSCs and PBMCs. Production of several MSC factors, including hepatocyte growth factor (HGF), TGF-beta, prostaglandin E2 (PGE2), and indoleamine 2, 3 dioxygenase (IDO), increased significantly in hAM-MSCs co-cultured with PBMCs. These results indicate that the immunomodulatory effects of hAM-MSCs may be associated with soluble factors (TGF-beta, HGF, PGE2, and IDO), suggesting that hAM-MSCs may have potential clinical use in regenerative medicine.
