Role of Endogenous Histamine on the Pathogenesis in the Endotoxin-Induced Acute Lung Injury.
- Author:
Tae Hyung KIM
1
;
Eun Kyung KIM
;
Ho Joo YOON
;
Mi Jung KIM
;
Jeoung Eun CHOI
;
Yeon Mok OH
;
Tae Sun SHIM
;
Chae Man LIM
;
Sang Do LEE
;
Woo Sung KIM
;
Dong Soon KIM
;
Won Dong KIM
;
Younsuck KOH
Author Information
- Publication Type:Original Article
- Keywords: Histamine; Acute lung injury (ALI); Endotoxin; Histamine receptor blockades
- MeSH: Acute Lung Injury*; Animals; Bronchoalveolar Lavage; Capillary Permeability; Cell Count; Endothelial Cells; Histamine*; Lung; P-Selectin; Peritoneum; Peroxidase; Rats; Rats, Sprague-Dawley; Receptors, Histamine; Trachea; Veins
- From:Tuberculosis and Respiratory Diseases 2003;54(1):91-103
- CountryRepublic of Korea
- Language:Korean
- Abstract: BACKGROUND: Histamine is widely distributed in the lung. It increases capillary permeability and the P-selectin expression on vascular endothelial cell surfaces. We studied the role of endogenous histamine on the pathogenesis of endotoxin-induced acute lung injury (ALI) in rats. METHODS: We instilled either normal saline (control group) or lipopolysaccharide (3 mg/Kg, LPS group) to tracheas of Sprague-Dawley rats. H1-receptor blocker (mepyramine, 10 mg/Kg, H1RB group), H2-receptor blocker (ranitidine, 10 mg/Kg, H2RB group), and H3-receptor blocker (thioperamide, 2 mg/Kg, H3RB group) were administered through vein or peritoneum along with intratracheal LPS administration. Statistical significance was accepted at p<0.05. RESULTS: LPS increases the histamine level in BAL fluid significantly at 2 h after the treatment compared with control group. LPS significantly increases protein concentration, PMN cell count in bronchoalveolar lavage (BAL) fluid, and myeloperoxidase (MPO) activity in the lung tissue at 6 h compared to control group. PMN cell count in BAL fluid and MPO activity in lung tissue were significantly lower in H2RB-group compared to LPS-group. However, protein concentration in BAL fluid showed no significant differences between the LPS alone and LPS with histamine receptor blockade. CONCLUSIONS: Endogenous histamine might be involved in the recruitment of PMNs in LPS-induced ALI via H2 receptor. However, its role in ALI would not be significant in this model.
