Induction of humoral responses to BHV-1 glycoprotein D expressed by HSV-1 amplicon vectors.
- Author:
Andrea Maria BLANC
1
;
Mabel Beatriz BEROIS
;
Lorena Magali TOME
;
Alberto L EPSTEIN
;
Juan Ramon ARBIZA
Author Information
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords: antibody response; BHV-1 gD; HSV-1 amplicon vectors; immunization
- MeSH: Animals; Antibodies, Viral/blood; Blotting, Western/veterinary; Cattle; Female; Genetic Vectors/*immunology; Herpesvirus 1, Bovine/genetics/*immunology; Herpesvirus 1, Human/genetics/*immunology; Immunity, Humoral/immunology; Immunization/methods/veterinary; Infectious Bovine Rhinotracheitis/*immunology/prevention & control/virology; Mice; Mice, Inbred BALB C; Neutralization Tests/veterinary; Specific Pathogen-Free Organisms; Viral Proteins/genetics/*immunology; Viral Vaccines/immunology
- From:Journal of Veterinary Science 2012;13(1):59-65
- CountryRepublic of Korea
- Language:English
- Abstract: Herpes simplex virus type-1 (HSV-1) amplicon vectors are versatile and useful tools for transferring genes into cells that are capable of stimulating a specific immune response to their expressed antigens. In this work, two HSV-1-derived amplicon vectors were generated. One of these expressed the full-length glycoprotein D (gD) of bovine herpesvirus 1 while the second expressed the truncated form of gD (gDtr) which lacked the trans-membrane region. After evaluating gD expression in the infected cells, the ability of both vectors to induce a specific gD immune response was tested in BALB/c mice that were intramuscularly immunized. Specific serum antibody responses were detected in mice inoculated with both vectors, and the response against truncated gD was higher than the response against full-length gD. These results reinforce previous findings that HSV-1 amplicon vectors can potentially deliver antigens to animals and highlight the prospective use of these vectors for treating infectious bovine rhinotracheitis disease.
