Interaction between chicken protein tyrosine phosphatase 1 (CPTP1)-like rat protein phosphatase 1 (PTP1) and p60v-src in v-src-transformed Rat-1 fibroblasts.
- Author:
Eun Joo JUNG
1
;
Choong Won KIM
Author Information
1. Department of Biochemistry, College of Medicine, and Research Institute of Life Science, Gyeongsang National University, Chinju, Korea. cwkim@gshp.gsnu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
oncogene protein pp60 (v-src);
phosphoprotein phosphatase;
protein interaction mapping;
protein-tyrosine-phosphatase
- MeSH:
Animals;
Blotting, Western;
Cell Line, Transformed;
Chickens;
Female;
Fibroblasts;
Oncogene Protein pp60(v-src)/*metabolism;
Phosphoprotein Phosphatase/genetics/*metabolism;
Precipitin Tests;
Protein Binding;
Protein-Tyrosine-Phosphatase/genetics/*metabolism;
Rabbits;
Rats;
Recombinant Fusion Proteins/genetics/metabolism
- From:Experimental & Molecular Medicine
2002;34(6):476-480
- CountryRepublic of Korea
- Language:English
-
Abstract:
CPTP1 is a nontransmembrane chicken protein tyrosine phosphatase having 92% sequence homology to the corresponding 321 amino acids of human protein tyrosine phosphatase 1B (HPTP1B). Using anti-CPTP1 antibody, we identified CPTP1-like rat PTP1 of 51 kappa Da in Rat-1 and v-src-transformed Rat-1 fibroblasts. Here we show that CPTP1-like rat PTP1 binds to p60v-src in vivo and CPTP1 also can associate with p60v-src in cell lysate of v-src- transformed Rat-1 fibroblasts. Interaction between HPTP1B-type PTPs, CPTP1-like rat PTP1 and CPTP1, and p60v-src was reduced by vanadate treatment for 13 h due to down regulation of the protein level of p60v-src in vivo. Interestingly, CPTP1-like rat PTP1 was coimmunoprecipitated with a 70-kappa Da protein which has a possibility to be tyrosine- phosphorylated by p60v-src in v-src-transformed Rat- 1 fibroblasts. These results suggest that HPTP1B- type PTPs may play an important role in p60src dependent signal pathway in eucaryotic cells.
