Clusterin expression and paclitaxel resistance in cervical cancer cell lines.
- Author:
Jae Eun SHIN
1
;
Eun Young SHIN
;
Yong Wook KIM
;
Dae Hoon KIM
;
Dong Choon PARK
Author Information
1. Department of Obstetrics and Gynecology, The Catholic University of Korea. dcpark@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Clusterin;
Cervical cancer;
Paclitaxel;
Drug resistance
- MeSH:
Blotting, Western;
Cell Line*;
Clusterin*;
Drug Resistance;
Inhibitory Concentration 50;
Paclitaxel*;
RNA, Small Interfering;
Transfection;
Uterine Cervical Neoplasms*
- From:Korean Journal of Obstetrics and Gynecology
2006;49(5):1060-1064
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: The purpose of this study was to evaluate whether clusterin expression is significantly correlated with paclitaxel resistance in cervical cancer cell lines. METHODS: The expressions of clusterin in cervical cancer cell lines were validated with western blotting. And subsequently, the correlation between clusterin expression levels and the IC50 of paclitaxel in several cervical cancer cell lines was evaluated. After transfection of clusterin siRNA into HeLaS3, which expressed high level of clusterin, their effect on chemo-resistance was evaluated by XTT assay. RESULTS: Clusterin expression levels were correlated with paclitaxel resistance in cervical cancer cell lines. And after transfection of clusterin siRNA into HeLa-S3 significantly decreased paclitaxel resistance (P<0.05). CONCLUSION: These findings suggest that clusterin expression may correlate with paclitaxel resistance.
