Apoptotic Cell Death in Experimental Retinal Vein Occlusion.
- Author:
Shin Goo KANG
1
;
Hum CHUNG
Author Information
1. Department of Ophthalmology, College of Medicine, Seoul National University.
- Publication Type:Original Article
- Keywords:
Apoptosis;
Ischemic injury;
Retinal ganglion cell;
Retinal vein occlusion;
TUNEL staining
- MeSH:
Animals;
Apoptosis;
Cell Death*;
DNA;
Electrophoresis;
In Situ Nick-End Labeling;
Microscopy, Electron;
Models, Animal;
Necrosis;
Neurons;
Rats;
Retinal Ganglion Cells;
Retinal Vein Occlusion*;
Retinal Vein*;
Retinaldehyde*;
Veins
- From:Journal of the Korean Ophthalmological Society
2000;41(3):576-584
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
We used an animal model of laser-induced retinal vein occlusion to study the temporal and spatial patterns of neuronal necrosis and apoptosis. After photodynamic retinal vein thrombosis with argon-green laser, rats were sacrificed at 6 hours, 12 hours, 1 day, 2 days, 3 days, 5 days, 7 days, and 14 days after vein occlusion. The temporal and spatial patterns of neuronal cell death were determined using light and electron microscopy, TdT-dUTP nick-end labeling[TUNEL]and DNA gel electrophoresis. The cells in retinal ganglion cell layer and inner nuclear layer showed both necrotic and apoptotic changes 12 hours after vein occlusion. The TUNEL positivity were detected at day 1 after vein occlusion and the number of positive cell increased until day 2, and decreased thereafter. DNA ladder pattern was observed 48 hours after vein occlusion by DNA gel electrophoreisis. These data demonstrated that retinal vein occlusion induces necrosis and apoptosis in the retinal ganglion cell and inner nuclear layer.
