Alum Directly Modulates Murine B Lymphocytes to Produce IgG1 Isotype.

Author: Bo Ra JIN ¹ ; Sun Jin KIM ; Jeong Min LEE ; Seong Ho KANG ; Hye Ju HAN ; Young Saeng JANG ; Goo Young SEO ; Pyeung Hyeun KIM
Author Information

1. Department of Molecular Bioscience, College of Biomedical Science, Kangwon National University, Chuncheon 200-701, Korea. phkim@kangwon.ac.kr
Publication Type:In Vitro ; Original Article
Keywords: Alum; IgG1; B lymphocyte; Isotype switch
MeSH: Alum Compounds; Aluminum Hydroxide; Animals; B-Lymphocytes; Cell Count; Enzyme-Linked Immunosorbent Assay; Enzyme-Linked Immunospot Assay; Humans; Hydroxides; Immunoglobulin Class Switching; Immunoglobulin G; Mice; Spleen; Vaccines
From:Immune Network 2013;13(1):10-15
CountryRepublic of Korea
Language:English
Abstract: Aluminum hydroxide (alum) is the most widely used adjuvant in human vaccines. Nevertheless, it is virtually unknown whether alum acts on B cells. In the present study, we explored the direct effect of alum on Ig expression by murine B cells in vitro. LPS-activated mouse spleen B cells were cultured with alum, and the level of isotype-specific Ig secretion, IgG1 secreting cell numbers, and Ig germ-line transcripts (GLT) were measured using ELISA, ELISPOT, and RT-PCR, respectively. Alum consistently enhanced total IgG1 production, numbers of IgG1 secreting cells, and GLTgamma1 expression. These results demonstrate that alum can directly cause IgG1 isotype switching leading to IgG1 production.