Effect of (-)-epigallocatechin-3-gallate on maintaining the periodontal ligament cell viability of avulsed teeth: a preliminary study.
10.5051/jpis.2011.41.1.10
- Author:
Im Hee JUNG
1
;
Jeong Ho YUN
;
Ah Ran CHO
;
Chang Sung KIM
;
Won Gyun CHUNG
;
Seong Ho CHOI
Author Information
1. Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry, Seoul, Korea. shchoi726@yuhs.ac.kr
- Publication Type:Original Article
- Keywords:
Epigallocatechin gallate;
Organ preservation;
Tooth replantation;
MTT formazan
- MeSH:
Animals;
Catechin;
Cell Survival;
Dogs;
Eosine Yellowish-(YS);
Formazans;
Hand;
Organ Preservation;
Periodontal Ligament;
Tea;
Tetrazolium Salts;
Thiazoles;
Tooth;
Tooth Avulsion;
Tooth Replantation
- From:Journal of Periodontal & Implant Science
2011;41(1):10-16
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Avulsed tooth can be completely recovered, if sound periodontal ligament (PDL) of tooth is maintained. Although a lot of storage solutions have been explored for the better storage of avulsed tooth, there is a shortcoming that the preservation time is much short. On the other hand, there has been studies that (-)-epigallocatechin-3-gallate (EGCG), the most abundant polyphenol in green tea, which is related to the anti inflammatory, antioxygenic, and antibacterial effects, allows the successful preservations of tissues and cells. This study evaluated the effect of EGCG on avulsed-teeth preservation of Beagle dogs for a period of time. METHODS: The atraumatically extracted teeth of Beagle dogs were washed and preserved with 0/10/100 microM of EGCG at the time of immediate, period 1 (4 days in EGCG-contained media and additional 1 day in EGCG-free media), period 2 (8 days in EGCG-contained media and additional 2 days in EGCG-free media) and period 3 (12 days in EGCG-contained media and additional 2 days in EGCG-free media). Then, the cell viabilities of preserved teeth was calculated by dividing optical density (OD) of 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay with OD of eosin assay to eliminate the measurement errors caused by the different tissue volumes. RESULTS: From the results, the immediately analyzed group presented the highest cell viability, and the rate of living cells on teeth surface decreased dependent on the preservation period. However, the 100 microM of EGCG-treated group showed statistically significant positive cell activity than EGCG-free groups throughout preservation periods. CONCLUSIONS: Our findings showed that 100 microM EGCG could maintain PDL cell viability of extracted tooth. These results suggest that although EGCG could not be a perfect additive for tooth preservation, it is able to postpone the period of tooth storage. However, further in-depth studies are required for more plausible use of EGCG.
