Effects of mixed leukocyte reaction, hydrocortisone and cyclosporine on expression of leukocyte adhesion molecules by endothelial and mesangial cells.
10.3346/jkms.1996.11.6.495
- Author:
Chun Gyoo IHM
1
;
Seong Pyo HONG
;
Jae Kyung PARK
;
Tae Won LEE
;
Byoung Soo CHO
;
Mun Ho YANG
;
Myung Jae KIM
Author Information
1. Department of Internal Medicine, College of Medicine, Kyung Hee University, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Alloreactive cells;
ICAM-1;
VCAM-1;
Hydrocortisone;
Cyclosporine
- MeSH:
Cells, Cultured;
Cyclosporine/*pharmacology;
Endothelium, Vascular/drug effects/*immunology;
Glomerular Mesangium/drug effects/*immunology;
Human;
Hydrocortisone/*pharmacology;
Intercellular Adhesion Molecule-1/*biosynthesis;
Interleukin-1/pharmacology;
Leukocytes/drug effects/*metabolism;
Lymphocyte Culture Test, Mixed;
Monocytes/drug effects/metabolism;
Tumor Necrosis Factor/pharmacology;
Vascular Cell Adhesion Molecule-1/*biosynthesis
- From:Journal of Korean Medical Science
1996;11(6):495-500
- CountryRepublic of Korea
- Language:English
-
Abstract:
We investigated the effects of mixed leukocyte reaction (MLR), hydrocortisone (HC) and cyclosporine A (CsA) on the expression of leukocyte adhesion molecules on the mesangial (MC) and endothelial cells (EnC). Cell surface enzyme immunoassay showed that INFnu, IL-1beta, or TNF alpha stimulated expression of ICAM-1, or VCAM-1 on MC after 24 hours. Flow cytometric analysis demonstrated that MLR supernatant induced a marked increase in mean fluorescence of or % of cells highly expressing intercellular adhesion molecule(ICAM)-1 or vascular cell adhesion molecule (VCAM)-1 on both cells after 24 hours (p<0.001). HC treatment(300 ng/ml) during MLR effectively inhibited MLR-induced upregulation of ICAM-1 and VCAM-1 on both cells (p<0.005). When MLR supernatant with HC was added to adhesion molecule assay, there was no inhibitory effect of HC on VCAM-1. CsA treatment (500 ng/ml) during MLR caused a modest decrease in upregulation of VCAM-1 on EnC (p<0.05), but had no effects on ICAM-1 on both cells. CsA directly decreased expression of VCAM-1 on MC. In conclusion, alloreactive lymphocytes and monocytes upregulate the expression of VCAM-1 and ICAM-1 on target cells probably by the mediation of cytokines. HC effectively prevents MLR-induced upregulation of VCAM-1 and ICAM-1. CsA does not increase the expression of VCAM-1 and ICAM-1.
