Comparison of Reprogramming Methods for Generation of Induced-Oligodendrocyte Precursor Cells.
10.4062/biomolther.2017.066
- Author:
Eun Hye LEE
1
;
Chang Hwan PARK
Author Information
1. Department of Biomedical Science, Graduate School, Hanyang University, Seoul 04763, Republic of Korea. chshpark@hanyang.ac.kr
- Publication Type:Original Article
- Keywords:
Direct conversion;
Oligodendrocyte;
iOPC;
Efficiency
- MeSH:
Animals;
Cell Transplantation;
Cell- and Tissue-Based Therapy;
Cellular Reprogramming;
Fibroblasts;
Methods*;
Mice;
Oligodendroglia;
Rats;
Stem Cells;
Transcription Factors;
Transplants
- From:Biomolecules & Therapeutics
2017;25(4):362-366
- CountryRepublic of Korea
- Language:English
-
Abstract:
Direct conversion by trans-differentiation is of growing interest in cell therapy for incurable diseases. The efficiency of cell reprogramming and functionality of converted cells are important considerations in cell transplantation therapy. Here, we compared two representative protocols for the generation of induced-oligodendrocyte progenitor cells (iOPCs) from mouse and rat fibroblasts. Then, we showed that induction of Nkx6.2, Olig2, and Sox10 (NOS) was more effective in mouse fibroblasts and that induction of Olig2, Sox10, and Zfp536 (OSZ) was more effective at reprogramming iOPCs from rat fibroblasts. However, OSZ-iOPCs did not show greater proliferation than NOS-induced cells. Because the efficiency of iOPCs generation appears to differ between cell species depending on transcription factors and culture conditions, it is important to select appropriate methods for efficient reprogramming.
