Role of TAZ in Lysophosphatidic Acid-Induced Migration and Proliferation of Human Adipose-Derived Mesenchymal Stem Cells.
10.4062/biomolther.2016.263
- Author:
Won Min MO
1
;
Yang Woo KWON
;
Il Ho JANG
;
Eun Jung CHOI
;
Sang Mo KWON
;
Jae Ho KIM
Author Information
1. Department of Physiology, Pusan National University School of Medicine, Yangsan 50612, Republic of Korea. jhkimst@pusan.ac.kr
- Publication Type:Original Article
- Keywords:
LPA;
TAZ;
Mesenchymal stem cells;
MEK;
ROCK
- MeSH:
Animals;
Fibroblasts;
Humans*;
Mesenchymal Stromal Cells*;
Mice;
Mice, Knockout;
Phosphorylation;
Receptors, Lysophosphatidic Acid
- From:Biomolecules & Therapeutics
2017;25(4):354-361
- CountryRepublic of Korea
- Language:English
-
Abstract:
Transcriptional co-activator with a PDZ-binding motif (TAZ) is an important factor in lysophosphatidic acid (LPA)-induced promotion of migration and proliferation of human mesenchymal stem cells (MSCs). The expression of TAZ significantly increased at 6 h after LPA treatment, and TAZ knockdown inhibited the LPA-induced migration and proliferation of MSCs. In addition, embryonic fibroblasts from TAZ knockout mice exhibited the reduction in LPA-induced migration and proliferation. The LPA1 receptor inhibitor Ki16425 blocked LPA responses in MSCs. Although TAZ knockdown or knockout did not reduce LPA-induced phosphorylation of ERK and AKT, the MEK inhibitor U0126 or the ROCK inhibitor Y27632 blocked LPA-induced TAZ expression along with the reduction in the proliferation and migration of MSCs. Our data suggest that TAZ is an important mediator of LPA signaling in MSCs in the downstream of MEK and ROCK signaling.
