Circulating Plasma and Exosomal microRNAs as Indicators of Drug-Induced Organ Injury in Rodent Models.
10.4062/biomolther.2016.174
- Author:
Young Eun CHO
1
;
Sang Hyun KIM
;
Byung Heon LEE
;
Moon Chang BAEK
Author Information
1. Department of Molecular Medicine, Kyungpook National University, Daegu 41944, Republic of Korea. mcbaek@knu.ac.kr
- Publication Type:Original Article
- Keywords:
miRNAs;
Exosomes;
Liver-specific injury;
N-acetyl cysteine;
Biomarkers
- MeSH:
Animals;
Biomarkers;
Exosomes;
Kidney;
Liver;
Mice;
MicroRNAs*;
Plasma*;
Polymerase Chain Reaction;
Rodentia*
- From:Biomolecules & Therapeutics
2017;25(4):367-373
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study was performed to evaluate whether microRNAs (miRNAs) in circulating exosomes may serve as biomarkers of drug-induced liver, kidney, or muscle-injury. Quantitative PCR analyses were performed to measure the amounts of liver-specific miRNAs (miR-122, miR-192, and miR-155), kidney-specific miR-146a, or muscle-specific miR-206 in plasma and exosomes from mice treated with liver, kidney or muscle toxicants. The levels of liver-specific miRNAs in circulating plasma and exosomes were elevated in acetaminophen-induced liver injury and returned to basal levels by treatment with antioxidant N-acetyl-cysteine. Circulating miR-146a and miR-206 were increased in cisplatin-induced nephrotoxicity and bupivacaine-induced myotoxicity, respectively. Taken together, these results indicate that circulating plasma and exosomal miRNAs can be used as potential biomarkers specific for drug-induced liver, kidney or muscle injury.
