Chemopreventive effects of garlic and mugwort mixture extract on Helicobacter pylori-associated mouse gastric carcinogenesis.
- Author:
So Young YOUM
1
;
Tae Wang KIM
;
Sang Kyung SHIN
;
Heon Sang JEONG
;
Jong Koo KANG
;
Tae Myoung KIM
;
Kil Ho KIM
;
Byeongwoo AHN
Author Information
1. College of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, Korea. bwahn@cbu.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
artemisia;
garlic;
gastric cancer;
Helicobacter pylori;
mouse
- MeSH:
Animals;
Artemisia*;
Atrophy;
beta Catenin;
Carcinogenesis*;
Garlic*;
Gastric Mucosa;
Gastritis;
Helicobacter pylori;
Helicobacter*;
Incidence;
Inflammation;
Medicine, Traditional;
Metaplasia;
Methylnitrosourea;
Mice*;
Mucins;
Stomach Neoplasms
- From:Korean Journal of Veterinary Research
2014;54(3):171-177
- CountryRepublic of Korea
- Language:English
-
Abstract:
Garlic and mugwort have long been used in traditional medicine to prevent various diseases. Several in vitro studies have reported protective efficacies of garlic and mugwort in cases of gastric cancer. In the present study, we investigated the cancer preventive effects of garlic and mugwort mixture extract (GME) in a Helicobacter (H.) pylori-associated gastric carcinogenesis mouse model. To induce gastric cancer, C57BL/6 mice were treated with N-methyl-N-nitrosourea and H. pylori. Various concentrations of GME (0, 100, 500, and 1,000 ppm) were then fed to the mice for 38 weeks, after which the tumor tissues were examined for histopathology, mucin histochemistry and beta-catenin. The incidence of gastric tumors was significantly lower in the highest dose GME-treated mice (46.7%) than control mice (85.7%) (p < 0.05). The multiplicity and size of tumors were also significantly reduced by GME feeding in a dose-dependent manner (p < 0.01). Furthermore, GME suppressed the H. pylori-associated chronic inflammation measured by histologic grading of H. pylori density, chronic gastritis, glandular atrophy and intestinal metaplasia in non-tumorous gastric mucosae. Our data suggest that GME suppresses gastric tumorigenesis via suppression of H. pylori-associated chronic inflammation.
