The Expression of Toll-like Receptors in Collagen-induced Arthritis.
- Author:
Jun Hee LEE
1
;
Soo Bong LEE
;
Geun Tae KIM
;
Sun RYU
;
Ju In KIM
;
Sun Hee LEE
;
Sung Il KIM
Author Information
1. Department of Internal Medicine, College of Medicine, Pusan National University, Busan, Korea. ksimd@pusan.ac.kr
- Publication Type:Original Article
- Keywords:
Toll-like receptor-2;
Toll-like receptor-4;
Toll-like receptor-9;
IL-17;
Collagen-induced arthritis
- MeSH:
Animals;
Arthritis;
Arthritis, Experimental*;
Arthritis, Rheumatoid;
Collagen Type II;
Interleukin-17;
Knee Joint;
Mice;
RNA, Messenger;
Toll-Like Receptors*
- From:The Journal of the Korean Rheumatism Association
2006;13(2):140-149
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To evaluate the expression of Toll-like receptor (TLR) 2, 4 and 9 and investigate the effects of IL-17 on the expression of TLRs in experimental rheumatoid arthritis (RA) model. METHODS: After induction of collagen-induced arthritis (CIA) by type II collagen in DBA1 mice, phosphate-buffered saline (PBS, PBS group) or IL-17 (IL-17 group) was injected to both knee joint at day 28 and 32. At day 35, mice were sacrificed and knee joints were isolated. Synovial mRNA expressions of TLR-2, 4 and 9 determined by real-time RT-PCR were compared among normal DBA1 mice (normal group), PBS and IL-17 group. RESULTS: Synovial TLR-2, 4, and 9 mRNA expressions of IL-17 and PBS group were significantly higher than normal group, and those of IL-17 group were higher than PBS group. CONCLUSION: Synovial TLR-2, 4 and 9 expression was enhanced in CIA and up-regulated by local overexpression of IL-17. These results suggest that TLRs play a roles on CIA and IL-17 induced aggravation of arthritis in CIA.
