Artificial oocyte activation in intracytoplasmic sperm injection cycles using testicular sperm in human in vitro fertilization.
10.5653/cerm.2015.42.2.45
- Author:
Hee Jung KANG
1
;
Sun Hee LEE
;
Yong Seog PARK
;
Chun Kyu LIM
;
Duck Sung KO
;
Kwang Moon YANG
;
Dong Wook PARK
Author Information
1. Laboratory of Reproductive Biology and Infertility, Cheil General Hospital and Women's Healthcare Center, Dankook University College of Medicine, Seoul, Korea. gypsyroadcom@hotmail.com
- Publication Type:In Vitro ; Original Article
- Keywords:
Artificial oocyte activation;
Calcium ionophore;
Intracytoplasmic sperm injection;
Pentoxifylline;
Testicular sperm extraction
- MeSH:
Calcium;
Calcium Signaling;
Embryonic Structures;
Fertilization;
Fertilization in Vitro*;
Hospitals, General;
Humans;
Infertility;
Male;
Oocytes*;
Pentoxifylline;
Pregnancy Rate;
Sperm Injections, Intracytoplasmic*;
Sperm Motility;
Spermatozoa*
- From:Clinical and Experimental Reproductive Medicine
2015;42(2):45-50
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: Artificial oocyte activation (AOA) is an effective method to avoid total fertilization failure in human in vitro fertilization-embryo transfer (IVF-ET) cycles. AOA performed using a calcium ionophore can induce calcium oscillation in oocytes and initiate the fertilization process. We evaluated the usefulness of AOA with a calcium ionophore in cases of total fertilization failure in previous cycles and in cases of severe male factor infertility patients with non-motile spermatozoa after pentoxifylline (PF) treatment. METHODS: The present study describes 29 intracytoplasmic sperm injection (ICSI)-AOA cycles involving male factor infertility at Cheil General Hospital from January 2006 to June 2013. Patients were divided into two groups (control, n=480; AOA, n=29) depending on whether or not AOA using a calcium ionophore (A23187) was performed after testicular sperm extraction-ICSI (TESE-ICSI). The AOA group was further split into subgroups according to sperm motility after PF treatment: i.e., motile sperm-injected (n=12) and non-motile sperm-injected (n=17) groups (total n=29 cycles). RESULTS: The good embryo rate (52.3% vs. 66.9%), pregnancy rate (20.7% vs. 52.1%), and delivery rate (10.3% vs. 40.8%) were lower in the PF/AOA group than in the control group. When evaluating the effects of restoration of sperm motility after PF treatment on clinical outcomes there was no difference in fertilization rate (66.6% vs. 64.7% in non-motile and motile sperm, respectively), pregnancy rate (17.6% vs. 33.3%), or delivery rate (5.9% vs. 16.7%) between the two groups. CONCLUSION: We suggest that oocyte activation is a useful method to ensure fertilization in TESE-ICSI cycles regardless of restoration of sperm motility after PF treatment. AOA may be useful in selected patients who have a low fertilization rate or total fertilization failure.
