Effect of laser-assisted multi-point zona thinning on development and hatching of cleavage embryos in mice.
10.5653/cerm.2015.42.2.51
- Author:
Young Seok LEE
1
;
Min Jung PARK
;
Sea Hee PARK
;
Ja Seong KOO
;
Hwa Sook MOON
;
Bo Sun JOO
Author Information
1. Department of Pediatric Cardiology, Dong-A University Hospital, Busan, Korea.
- Publication Type:Original Article
- Keywords:
Blastocyst formation;
Hatching;
Laser-assisted zona thinning;
Multipoint laser-assisted zona thinning
- MeSH:
Animals;
Blastocyst;
Embryonic Structures*;
Female;
Herpes Zoster*;
Humans;
Mice*;
Morula;
Zona Pellucida
- From:Clinical and Experimental Reproductive Medicine
2015;42(2):51-57
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: This study aimed to examine the effect of laser-assisted zona thinning (LAZT) at one or four-points on the blastocyst formation and hatching process in mice with respect to female age. METHODS: Eight-cell or morula embryos collected from superovulated C57BL female mice with different ages (6-11 and 28-31 weeks) were treated with LAZT at one-point (LAZT1) or four-points (LAZT4). The zona pellucida was thinned to more than 70% of its initial thickness by making two holes of 15-20 microm. RESULTS: In the young mice, LAZT resulted in a significant increase in early hatching and hatching rates compared to the control group (p<0.05). However, in the old mice, LAZT significantly increased blastocyst formation as well as early hatching and hatching compared to the controls (p<0.05). These effects were more remarkable in LAZT4 than in LAZT1 and in aged mice than in young ones. CONCLUSION: These results show that multi-point LAZT leads to a significant improvement of blastocyst formation and hatching in mice compared to controls.
