Effect of Stress on the Expression of Rho-Kinase and Collagen in Rat Bladder Tissue.
10.4111/kju.2010.51.2.132
- Author:
Hana YOON
1
;
Donghyun LEE
;
Kyemin CHUN
;
Hyunsuk YOON
;
Jaeyeong YOO
Author Information
1. Department of Urology, Medical Research Center, Ewha Womans University School of Medicine, Seoul, Korea. wowhana@ewha.ac.kr
- Publication Type:Original Article
- Keywords:
Physiology;
Urinary bladder
- MeSH:
Animals;
Collagen;
Collagen Type I;
Collagen Type III;
Contracts;
Dopamine;
Estrogens;
Female;
Humans;
Muscle, Smooth;
Rats;
Rats, Sprague-Dawley;
Relaxation;
rho-Associated Kinases;
Testosterone;
Urinary Bladder;
Urination
- From:Korean Journal of Urology
2010;51(2):132-138
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: We examined the effect of stress on the pathophysiology of bladder stability in terms of enzyme levels, Rho-kinase, and bladder relaxation. MATERIALS AND METHODS: A total of 48 female Sprague-Dawley rats were studied in scheduled stress environments for 7, 14, and 28 days; 24 rats were in the control group and 24 rats were in the test (stressed) group. RESULTS: Estrogen decreased significantly whereas testosterone and dopamine increased significantly in the stress group (p<0.05). Rho-kinase was significantly increased in the rats exposed to stress stimuli for 14 days (p<0.05). Collagen types I and III in the bladder tissue were significantly higher in rats exposed to stress for 14 days and 28 days (collagen type I in the 14-day group, p<0.01; collagen type I in the 28-day group, p<0.05; collagen type III in the 14-day and 28-day groups, p<0.05). Voiding frequency increased significantly as the duration of stress exposure was prolonged, in addition to a significant decrease in volume per voiding (p<0.05). CONCLUSIONS: The changes observed in micturition pattern, factors that contribute to smooth muscle contraction, and relaxation in the female rat bladder support the hypothesis that stress affects bladder stability.
