Cyclooxgenase-2 Expression in Chronic Inflammatory Bowel Disease:Comparing of Immunohistochemical Stain to Real-time PCR.
- Author:
Young Sook PARK
1
;
Nam In KIM
;
Jun Seok LEE
;
Jeoung Ho CHOI
;
Chae Young IM
;
Dae Won JUN
;
Yun Ju JO
;
Seung Hwan KIM
;
Byung Kwan SON
;
Won Mee LEE
;
Eun Kyoung KIM
Author Information
1. Department of Internal Medicine, Eulji University School of Medicine, Eulji Medical Center, Seoul, Korea. pys1109@eulji.ac.kr
- Publication Type:Original Article
- Keywords:
Cyclooxygenase 2;
Inflammatory Bowel Diseases;
Immunohistochemistry;
Polymerase Chain Reaction
- MeSH:
Colitis;
Colitis, Ulcerative;
Colon;
Crohn Disease;
Cyclooxygenase 2;
Humans;
Immunohistochemistry;
Inflammation;
Inflammatory Bowel Diseases;
Mucous Membrane;
Polymerase Chain Reaction;
Real-Time Polymerase Chain Reaction
- From:Intestinal Research
2008;6(2):128-134
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND/AIMS: This study compared the results of cyclooxygenase-2 (COX-2) expression in inflammatory bowel disease and tuberculous colitis as evident by immunochemical staining and real time polymerase chain reaction (PCR). METHODS: Patients with ulcerative colitis (n=18), Crohn's disease (n=7), tuberculous colitis (n=7) and 10 normal controls were included. Biopsied colonic mucosa was simultaneously used for immunohistochemical staining and real time PCR. RESULTS: Patients with inflammatory bowel disease and tuberculous colitis showed high COX-2 expression by both methods compared to the normal controls. In Crohn's disease patients, the real time PCR value correlated well staining grade; this correlation was not evident in ulcerative colitis patients. In real time PCR, grossly normal colonic mucosa in ulcerative colitis also showed higher expression of COX-2 than normal mucosa. CONCLUSIONS: Real time PCR value of COX-2 is more representative of inflammation state in inflammatory bowel disease than the value from immunohistochemical staining.
