Prenatal Development of Human Lip with Immunohistochemical Study.
- Author:
Su Jung HONG
1
;
Young Joon LEE
;
Yeon Sook KIM
;
Suk Keun LEE
;
Je G CHI
Author Information
1. Department of Oral Pathology, Kangnung National University College of Dentistry, Gangneung, Korea. sklee@knusun.kangnung.ac.kr
- Publication Type:Original Article
- Keywords:
Fetal Development-Lip-Immunohistochemistry
- MeSH:
Antibodies;
Cadherins;
Epithelium;
Fetus;
Formaldehyde;
Humans*;
Immunohistochemistry;
Keratins;
Lip*;
Pregnancy;
Proliferating Cell Nuclear Antigen;
S100 Proteins;
Tenascin;
Tissue Inhibitor of Metalloproteinase-2
- From:Korean Journal of Pathology
2002;36(4):212-221
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: This study is aimed to elucidate the developmental pattern of human fetal lip by histological and immunohistochemical examinations. METHODS: Totally 231 normal human lip tissues obtained from autopsied fetuses were fixed with 10% buffered formalin, sectioned in cross and longitudinal directions, routinely stained for H&E and performed for immunohistochemistry with antibodies of S-100 protein, proliferating cell nuclear antigen (PCNA), transglutaminase C (TGase-C), metalloproteinase (MMP)-3, MMP-10, tenascin, KL1, K8.12, E-cadherin, tissue inhibitors of matrix metalloproteinase (TIMP)-1, TIMP-2 and total keratin (TK). RESULTS: The lip structure first appeared as an orifice of stomodeum around the 7-8th week of gestation, and a major structure of the midface was observed by the 11-12th week. As the squamous epithelium of the lip became thick and was keratinized, the vermilion border became distinguished in the 15-16th week, and the lip structure was almost completed with the presence of orbicularis oris muscle in the lingual side of vermilion border by the 17-18th week. Immunohistochemically, the vermilion border showed strong reactions for tenascin, E-cadherin and MMP-3 and increased positivity for PCNA, cytokeratins (TK, KL1, K8.12), and TGase-C. CONCLUSIONS: With the above findings we suppose that the cytodifferentiation of vermilion border epithelium plays an important role for the development of human fetal lip.
