Immunologic reactivity of a lipopolysaccharide-protein complex of type A Pasteurella multocida in mice.
- Author:
Hyo Ik RYU
;
Chul Joong KIM
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
P. multocida;
LPS-protein complex;
Immunogenicity
- MeSH:
Animals;
Antibodies, Bacterial/blood/immunology;
Bacterial Proteins/chemistry/*immunology;
Chemical Fractionation;
Hypersensitivity, Delayed;
Immune Sera/immunology;
Immunity, Cellular;
Immunization, Passive;
Lipopolysaccharides/chemistry/*immunology;
Lymphocyte Activation;
Mice;
Pasteurella Infections/immunology/*prevention & control;
Pasteurella multocida/*chemistry/immunology;
Salmonella Infections, Animal/immunology/prevention & control;
Salmonella enteritidis/growth & development/immunology;
Spleen/cytology/immunology/microbiology
- From:Journal of Veterinary Science
2000;1(2):87-95
- CountryRepublic of Korea
- Language:English
-
Abstract:
The immunologic reactivity of a lipopolysaccharide (LPS)-protein complex isolated from a potassium thiocyanate extract of a Pasteurella multocida (capsular type A and somatic type 3) strain was evaluated in mice. The LPS-protein complex provided 100% protection in mice against a challenge with the homologous strain. However, when the complex was fractionated into LPS and protein moieties by phenol-water treatment, both components lacked immunogenicity. The complex and extracted components were mitogenic for mouse B lymphocytes with the protein moiety the most active. Although immune serum against the LPS-protein complex protected mice against challenge thereby indicating a role for humoral immunity, the LPS-protein complex of P. multocida was also found to induce cell-mediated immunity. This cell-mediated immunity was demonstrated in mice immunized with the complex by: (1). mitogenic responses of T lymphocytes, (2). induction of delayed type hypersensitivity reaction in the hind footpads, and (3). enhanced resistance to challenge infection with Salmonella enteritidis.
