Evaluation of expression patterns of feline CD28 and CTLA-4 in feline immunodeficiency virus (FIV)-infected and FIV antigen-induced PBMC.
- Author:
In Soo CHOI
;
Han Sang YOO
;
Ellen W COLLISSON
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
- Keywords:
CD28;
CTLA-4;
FIV infection;
PBMC;
competitive PCR.
- MeSH:
Animals;
Antigens, CD;
Antigens, CD28/*biosynthesis;
Antigens, Differentiation/*biosynthesis;
Antigens, Viral/*immunology;
Cats;
Cell Survival;
Cells, Cultured;
Gene Expression;
Immunodeficiency Virus, Feline/immunology/*physiology;
Leukocytes, Mononuclear/immunology/*virology;
Polymerase Chain Reaction/veterinary
- From:Journal of Veterinary Science
2000;1(2):97-103
- CountryRepublic of Korea
- Language:English
-
Abstract:
It is known that CD28, a positive costimulatory receptor, plays a very important role in inducing the optimal stimulation of T lymphocytes. CTLA-4 (CD152), however, acts as a negative regulator in T lymphocyte activation. The effect of an feline immunodeficiency virus (FIV) infection on the expression of feline CD28 and CTLA-4 was studied with FIV-infected and uninfected peripheral blood mononuclear cells (PBMC) using a competitive PCR assay. The nature of CD28 and CTLA-4 expression was also examined with fresh and antigen-stimulated PBMC. FIV infection induced a lower expression of CD28, but a higher expression of CTLA-4 in the infected PBMC than in the uninfected PBMC. Relatively high levels of CD28 expression were demonstrated in both the fresh and the antigen-stimulated PBMC. The expression level of CTLA-4 in the freshly isolated PBMC was rather low, however, FIV antigen stimulation induced a relatively high expression of CTLA-4 in feline PBMC.
