Induction of Metallothionein and Manganese-Containing Superoxide Dismutase by Paraquat in Peripheral White Blood Cells of Patients with Gastric Cancer.
- Author:
Sung Pyo MOON
1
;
Cheol Hee CHOI
;
Yoon Jung CHA
;
Jung Hwan CHANG
;
Cheong Yong KIM
;
Young Don MIN
Author Information
1. Department of Surgery, College of Medicine, Chosun University, Kwangju, Korea.
- Publication Type:Original Article
- Keywords:
Gastric cancer;
Metallothionein;
Mn-SOD;
Paraquat
- MeSH:
Aging;
Antioxidants;
Carcinogenesis;
Culture Media;
DNA Damage;
Female;
Humans;
Leukocytes*;
Male;
Metallothionein*;
Paraquat*;
Reactive Oxygen Species;
RNA, Messenger;
Stomach Neoplasms*;
Superoxide Dismutase*;
Superoxides*;
Volunteers
- From:Journal of the Korean Surgical Society
2000;59(6):751-758
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: It is known that deficiency of metallothionein (MT) and manganese-containing superoxide dismutase (Mn-SOD), scavengers of reactive oxygen species, results in aging and carcinogenesis by inducing DNA damage. Paraquat can produce reactive oxygen species and induce antioxidants in human. In this study, an attempt was made to verify the relation between gastric carcinogensis and the induction rates of these antioxidants. METHODS: Peripheral blood of 24 randomly selected patients with gastric cancer, who were treated at Chosun University Hospital between Febuuary 1999 and December 1999, was examined. 3 male and 3 female patients in each decade from 40 to 70 years were selected. Twenty-four (24) volunteers with no laboratory, chemical, radiologic and endoscopic abnormalities during the same period were used for the normal control group. White blood cells were isolated from peripheral blood and incubated in culture media, including paraquat, for 18 hours at 37oC. MT and Mn-SOD mRNA expressions were examined using the reverse transcription-polymerase chain reaction (RT-PCR) method. RESULTS: The induction rates of MT and Mn-SOD mRNA in the cancer group due to paraquat were lower than those in the control group. Also, the rates decreased in both groups with age. CONCLUSION: The inducibilities of MT and Mn-SOD mRNA by paraquat may play a role in the carcinogenesis of gastric cancer and in the aging process. Based on this result, patients with a high risk of gastric cancer should be screened actively for early detection.
