The Change of Serum Level of Xanthine Oxidase Activity, Glutathione and Malonyldialdehyde following Renal Ischemia/Reperfusion Injury in the Rat.
- Author:
In Soo BAE
1
;
Hee Chang JUNG
;
Tong Choon PARK
Author Information
1. Department of Urology, College of Medicine, Yeungnam University, Taegu, Korea.
- Publication Type:Original Article
- Keywords:
mia/Reperfusion injury;
Oxygen free radicals;
Serum;
Renal function
- MeSH:
Animals;
Glutathione*;
Humans;
Lipid Peroxidation;
Male;
Malondialdehyde*;
Oxygen;
Rats*;
Rats, Sprague-Dawley;
Renal Artery;
Xanthine Oxidase*;
Xanthine*
- From:Korean Journal of Urology
1998;39(2):105-109
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: It has been suggested in our previous study that the level of xanthine oxidase(XO) activity, glutathione(GSH) and malonydialdehyde(MDA) in renal tissue following renal ischemia/reperfusion(I/R) could be used as marker of oxidant stress. Present study was undertaken to investigate the serum level of XO activity. GSH and MDA following renal I/R and to elucidate potential use of the serum level of GS H and MDA as makers of renal function following I/R injury. MATERIALS AND METHODS: Male Sprague-Dawley rats(200-250gm) were divided into 3 groups : group A - occlusion of bilateral renal arteries for 60 min, group B - pretreatment allopurinol(20mg/Kg). a radical scavenger, plus occlusion of renal arteries, and group C(control group) - sham operation. In group A and B, recirculation of renal arteries were performed for 30min. XO activity, xanthine dehydrogenase(XD) type conversion ratio, level of GSH and MDA were measured from venous blood. RESULTS: Both XO activity(nM/mg/min) and XD type conversion ratio(%) were increased in group A(XO; 0.173+/-0.012, XD; 60.44+/-4.32) and decreased in group B(XO; 0.136+/-0.01, XD; 45.40+/-4.78) compared to control group(XO; 0.153+/-0.012, XD; 46.93+/- 3.45). The level of GSH(microM/g tissue), a scavenger of oxygen free radical(OFR), was also decreased in group A(0.130+/-0.021) compared to group B(0.179+/-0.021) and control group(0.186+/-0.017). In addition, the level of MDA(nM/g tissue), which is a stable end product of lipid peroxidation, was significantly increased in group A(0.076+/-0.006) compared to group B(0.057+/-0.005) and control group(0.053+/-0.004). CONCLUSIONS: From these results, it is suggested that renal I/R injury is highly correlated with the production of OFR. Furthermore. the serum levels of MDA and GSH might be used as early markers of oxidant stress in association with renal I/R injury.
