Allele Frequencies of Apo(a) Pentanucleotide(TTTTA) Repeat Polymorphism.
- Author:
Sang Hyun HWANG
1
;
Woo Yang KIM
;
Sail CHUN
;
Won Ki MIN
Author Information
1. Department of Clinical Pathology, Asan Medical Center and University of Ulsan College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Apolipoprotein(a);
Lipoprotein(a);
Pentanucleotide repeat polymorphism;
Met/Thr polymorphism;
Polymorphism
- MeSH:
Adult;
Alleles*;
Apoprotein(a);
Electrophoresis, Polyacrylamide Gel;
Enzyme-Linked Immunosorbent Assay;
Gene Frequency*;
Genotype;
Humans;
Immunoblotting;
Kringles;
Linear Models;
Lipoprotein(a);
Lipoproteins;
Microsatellite Repeats;
Polymerase Chain Reaction
- From:Korean Journal of Clinical Pathology
2000;20(3):268-274
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Lipoprotein(a) [Lp(a)] is an atherogenic lipoprotein that is assembled from a low density lipoprotein(LDL) and apolipoprotein(a) [apo(a)]. The variations in Lp(a) concentration tend to be inversely related to the number of kringle IV in apo(a) gene, but other polymorphisms [pentanucleotide(TTTTA) repeats, +93 C/T polymorphism, and Met/Thr polymorphism] of the apo(a) gene are also seems to be related to Lp(a) concentrations. The purpose of this study was to investigate the association of the pentanucleotide repeat polymorphism(PNRP) and Met/Thr polymorphism with the Lp(a) concentrations. METHODS: We studied 197 healthy adults. For genotype analysis of the PNRP and the Met/Thr polymorphism, PCR was performed. Apo(a) phenotyping was performed by SDS-PAGE and immunoblotting. The Lp(a) concentrations were measured by ELISA method. More than two groups were compared using the Kruskal Wallis one-way analysis test. To establish a relationship between gene polymorphisms and Lp(a) concentrations, The linear regression test was performed. RESULTS: Mean Lp(a) concentration was 25.3+/-21.5 mg/dL. Allele frequencies for PNRP, subjects with 8/8 genotype were 114(57.9%) and most frequently observed. The Lp(a) concentrations showed the tendency to decrease as the sum of alleles of PNR increased. Subjects with Met/Thr genotype were 119(60.4%), Met/Met genotype were 71(36.0%) and Thr/Thr genotype were 7(3.6%). CONCLUSIONS: For PNRP, subjects with 8/8 genotype were 114(57.9%) and 8/8 genotype was the most frequently observed. Met/Thr genotype was most frequently observed.
