Proteomic applications of surface plasmon resonance biosensors: analysis of protein arrays.
- Author:
Jong Seol YUK
1
;
Kwon Soo HA
Author Information
1. Department of Molecular and Cellular Biochemistry, Nano-bio Sensor Research Center, Kangwon National University School of Medicine, Chuncheon, Gangwon-do, South Korea. ksha@kangwon.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't ; Review
- Keywords:
biosensing techniques;
microscopy;
atomic force;
protein array analysis;
proteome;
proteomics;
surface plasmon resonance
- MeSH:
Animals;
*Biosensing Techniques;
Humans;
Protein Array Analysis;
*Proteomics;
Research Support, Non-U.S. Gov't;
*Surface Plasmon Resonance/instrumentation/methods
- From:Experimental & Molecular Medicine
2005;37(1):1-10
- CountryRepublic of Korea
- Language:English
-
Abstract:
Proteomics is one of the most important issues in the post-genomic area, because it can greatly contribute to identifying protein biomarkers for disease diagnosis and drug screening. Protein array is a key technology for proteome researches and has been analyzed by various methods including fluorescence, mass spectrometry, atomic force microscopy and surface plasmon resonance (SPR). SPR biosensor is a promising technology in proteomics, since it has various advantages including real-time measurement of biomolecular interactions without labeling and the simple optical system for the device. SPR biosensors have a strong potential for analyzing proteomes by SPR imaging and SPR spectroscopic imaging, even though the challenge is to produce proteins on a proteomic scale.
