Molecular Epidemiologic Analysis of Legionella pneumophila by Infrequent-Restriction-Site Polymerase Chain Reaction.
- Author:
Jeong Man KIM
1
;
Kyeong Hee KIM
;
Eun Ju SONG
;
Sun Min LEE
;
Eun Yup LEE
;
Eun Hee PARK
;
Chulhun L CHANG
Author Information
1. Department of Laboratory Medicine, Dong-A University College of Medicine, Korea.
- Publication Type:Original Article
- Keywords:
Legionella pneumophila;
Infrequent-restriction-site polymerase chain reaction;
Molecular strain typing
- MeSH:
Busan;
Humans;
Japan;
Legionella pneumophila*;
Legionella*;
Legionellosis;
Molecular Typing;
Polymerase Chain Reaction*
- From:Korean Journal of Clinical Microbiology
2006;9(1):24-29
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The frequent outbreak of legionellosis makes it critical to identify infection sources for the prevention and blockade of transmission of the disease. METHODS: Thirty-one strains of Legionella pneumophila isolated from the cooling towers of big buildings in Busan and Gyungsangnamdo Province areas, 12 strains of L. pneumophila from patients in Japan, and one type strain (L. pneumophila ATCC 33152) were used for molecular strain typing by using an infrequent-restriction-site polymerase chain reaction (IRS-PCR). RESULTS: Each strain revealed to have 7-16 bands of 200-1000 bp size. All 44 strains showed band patterns different from each other, except two strains sharing 90% homology. CONCLUSION: The molecular typing of Legionellaby IRS-PCR is an excellent and rapid method for discriminating strains; therefore, it should be useful in demonstrating the identity of possible outbreak strains.
