Influenza virus-induced airway hyperreactivity in rats.
- Author:
Hye Kyung LEE
1
Author Information
1. Department of Pharmacology, Chonbuk National University Dental School, Chonju, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
Influenza virus;
airway hyperreactivity;
acetylcholine;
bronchoconstriction
- MeSH:
Acetylcholine;
Airway Resistance;
Animals;
Bronchoconstriction;
Compliance;
Humans;
Influenza, Human*;
Mechanics;
Models, Animal;
Muscles;
Orthomyxoviridae;
Rats*
- From:Journal of Asthma, Allergy and Clinical Immunology
2000;20(4):633-640
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVE: Studies indicate that humans infected with influenza virus manifest a post-virus increase in airway responsiveness. Therefore, whether influenza virus would cause airway hyperreactivity was investigated. SUBJECT AND METHOD: Rats were infected with rat-adapted influenza virus via nasal instillation. On days 1, 2, 3, 5, 7 and 9 post-virus inoculation, pulmonary mechanics were measured during an intravenous challenge with acetylcholine (ACh, 100-140 mg/ml saline). Cumulative dose-response functions were obtained by doubling the rate of the infusion every 2 minutes. RESULTS: ACh increased airway resistance and decreased dynamic compliance in a dosedependent manner. The airway responses to ACh was enhanced in most animals challenged 3 days after the inoculation. About half of the animals challenged 2 and 5 days after the virus inoculation showed hyperresponsiveness to ACh. No airway hyperresponsiveness was noted in animals on day 1 or 9 post-virus exposure. Tracheal muscle rings were isolated from infected, thus hyperresponsive animals and the contractile force to ACh and KCl was examined in vitro, where their dose-response characteristics were similar to those of muscles from controls. CONCLUSION: The rat model for influenza virus-induced airway hyperreactivity was developed, which manifested a post-virus increase in airway responsiveness.
